+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of 2S and 7S storage proteins in embryos of oil palm

Characterization of 2S and 7S storage proteins in embryos of oil palm

Plant Science (Shannon) 122(2): 141-151

Storage proteins that accumulated during oil palm embryo development were extracted, purified and characterized. Only water- and low-salt-soluble proteins, with respective sedimentation coefficients of 2S and 7S, were detected in mature embryos. After purification by gel filtration, the various protein classes identified were characterized by electrophoresis and amino acid composition analysis. The 2S proteins comprise polypeptides of 22 kD and 19 kD, which are acidic (pI lt 6) and basic (pI gt 9) respectively. The 7S proteins predominate and are heterogeneous oligomers (Mr of 156 and 201 kD), comprising a polypeptide triplet of Mr between 45 and 65 kD with no disulphide bonds. Their amino acid composition is broadly similar to those of the 7S proteins of other monocotyledon embryos, but differs from those of the legume 7S vicilins. Histological examinations and electrophoresis showed that the 2S and 7S proteins appeared at the third month after fertilization, and no qualitative changes were detected up to the sixth month of embryo development. The characterization of the embryo storage proteins of oil palm is discussed with reference to legume, cereal and palm seed storage proteins. This study will enable further investigation of storage protein synthesis during somatic embryogenesis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002775111

Download citation: RISBibTeXText

DOI: 10.1016/s0168-9452(96)04555-4

Related references

Evaluation of somatic embryos of interior spruce characterization and developmental regulation of storage proteins. Physiologia Plantarum 82(4): 624-632, 1991

Identification and characterization of storage proteins in zygotic and somatic embryos of geranium (Pelargonium x hortorum). Journal of the American Society for Horticultural Science 125(4): 525-529, 2000

Seed storage proteins in developing somatic embryos of alfalfa: defects in accumulation compared to zygotic embryos. Journal of Experimental Botany 45(275): 699-708, 1994

Effect of desiccation and storage temperature on the conservation of cultures on oil palm somatic embryos. Cryo Letters 15(2): 85-90, 1994

Accumulation pattern and identification of seed storage proteins in zygotic embryos of Pinus strobus and in somatic embryos from different maturation treatments. Physiologia Plantarum 121(4): 682-690, 2004

Studies on the in vitro culture of oil palm embryos. IV. Effects of seed moisture content and storage time. Oleagineux. 23: 233-7. bibl. 15, 1968

Research on the culture in vitro of oil palm m embryos elaeis guineensis m iv effect of the water content of the kernels and of the length of storage. Oleagineux: 233-237, 1968

Effect of osmoticum and fluridone on synthesis of storage proteins and LEA proteins of peanut embryos cultured in vitro. Acta Phytophysiologica Sinica 22(4): 368-372, 1996

Developments in the storage of embryos in France and the limitations of the laws of bioethics. Analysis of procedures in 17 storage centres and the destiny of stored embryos. Medicine and Law 21(3): 587-604, 2002

Characterization of seed storage proteins in Populus and their homology with Populus vegetative storage proteins. Tree Physiology 16(10): 833-840, 1996

Some observations on stress tolerance in sago palm (Metroxylon sagu) and oil palm (Elaeis guineensis) using in vitro cultured embryos. Towards greater advancement of the sago industry in the ' 90s Proceedings of the fourth international sago symposium, 6-9 August 1990, Kuching, Sarawak, Malaysia: 64, 1991

Synthesis and storage of microtubule proteins by sea urchin embryos. Journal of Cell Biology 50(2): 516-527, 1971

Changes in ribosomal proteins in barley embryos during the storage of grain in the ear. Hodowla Roslin, Aklimatyzacja i Nasiennictwo 26(3): 245-252, 1982