+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of Arabidopsis thaliana cDNAs that render yeasts tolerant toward the thiol-oxidizing drug diamide



Characterization of Arabidopsis thaliana cDNAs that render yeasts tolerant toward the thiol-oxidizing drug diamide



Proceedings of the National Academy of Sciences of the United States of America 92(23): 10580-4



Diamide oxidizes cellular thiols and induces oxidative stress. To isolate plant genes which may, when overexpressed, increase tolerance of plants toward oxidative damage, an in vivo diamide tolerance screening in yeasts was used. An Arabidopsis cDNA library in a yeast expression vector was used to transform a yeast strain with intact antioxidant defense. Cells from approximately 10(5) primary transformants were selected for resistance to diamide. Three Arabidopsis cDNAs which confer diamide tolerance were isolated. This drug tolerance was specific and no cross tolerance toward hydroperoxides was found. One cDNA (D3) encodes a polypeptide which has an amino-terminal J domain characteristic of a divergent family of DnaJ chaperones. Another (D18) encodes a putative dTDP-D-glucose 4,6-dehydratase. Surprisingly, the third cDNA (D22) encodes a plant homolog of gamma-glutamyltransferases. It would have been difficult to predict that the expression of those genes would lead to an improved survival under conditions of depletion of cellular thiols. Hence, we suggest that this cloning approach may be a useful contribution to the isolation of plant genes that can help to cope with oxidative stress.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775125

Download citation: RISBibTeXText

PMID: 7479844

DOI: 10.2307/2368831


Related references

Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. Journal of Biological Chemistry 270(44): 26224-26231, 1995

Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana. FEBS Letters 351(2): 257-262, 1994

Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana. Plant Molecular Biology 35(4): 483-495, 1997

Characterization and Expression of Two cDnas Encoding Carbonic Anhydrase in Arabidopsis thaliana. Plant Physiology 105(2): 707-713, 1994

Isolation and characterization of cDNAs encoding imidazoleglycerolphosphate dehydratase from Arabidopsis thaliana. Plant Physiology 105(2): 579-583, 1994

Characterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis thaliana. Plant Physiology 105(2): 707-713, 1994

Isolation and Characterization of cDnas Encoding Imidazoleglycerolphosphate Dehydratase from Arabidopsis thaliana. Plant Physiology 105(2): 579-583, 1994

Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase. Proceedings of the National Academy of Sciences of the United States of America 92(6): 2328-2332, 1995

Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes. Plant Journal 6(1): 113-121, 1994

Characterization of two novel seed-specific cDNAs identified in immature Arabidopsis thaliana seeds. Plant Physiology (Rockville) 111(2 Suppl. ): 158, 1996

Cloning and characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thaliana. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 21(2): 279-289, 1993

Stimulation of septation in mitochondria by diamide a thiol oxidizing agent. Cell & Tissue Research 203(2): 291-300, 1979

Glutathione. VII. Differentiation among substrates by the thiol-oxidizing agent, diamide. Biochimica et Biophysica Acta 264(1): 39-44, 1972

Characterization of two cDNAs (ERD10 and ERD14) corresponding to genes that respond rapidly to dehydration stress in Arabidopsis thaliana. Plant & Cell Physiology 35(2): 225-231, 1994

Effect of the thiol-oxidizing agent diamide on the growth of Escherichia coli. Journal of Bacteriology 101(3): 1092-1093, 1970