+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of Rhizoctonia solani AG 2 isolates causing bare patch in field grown tulips in the Netherlands

Characterization of Rhizoctonia solani AG 2 isolates causing bare patch in field grown tulips in the Netherlands

European Journal of Plant Pathology 103(3): 265-279

During a spring survey in 1991, 130 isolates of R. solani were collected in 25 commercial flower bulb fields from diseased plants occurring in bare patches. On the basis of hyphal fusion frequency and pathogenicity to flower bulbs, tulip isolates, were provisionally assigned to AG 2-t to distinguish these isolates from AG 2-1 isolates which were non-pathogenic to bulbs. Hyphal fusion frequency of a subgroup of 7 AG 2-t isolates was highly variable when paired with 7 AG 2-1 isolates (2-75%), thus making assignment of AG 2-t isolates to AG 2-1 inconclusive. The mean hyphal fusion frequency among AG 2-t isolates was 65% (+-6%) indicating AG 2-t to be a relatively homogeneous group. Hyphal fusion frequency among AG 2-1 isolates was highly variable with a mean 51% (+-25%) indicating AG 2-1 to be a heterogeneous group. The optimum growth temperature for AG 2-5 and AG 2-1 isolates on malt peptone agar was 20-25 degree C. The host range of AG 2-t and two AG 2-1 isolates comprised tulip, iris, hyacinth and lily at both and 18 degree C, and cruciferous, sugarbeet and lettuce seedlings at 18 degree C. Six other AG 2-1 isolates were pathogenic to cruciferous seedlings, but not to any of the bulbous crops. The tested narcissus, Tagetes patula, tomato, potato, wheat, leek and maize cultivars were not susceptible to AG 2-t and AG 2-1 isolates. Statistical analysis using a proportional-odds model revealed significant differences in aggressiveness between R. solani AG 2-t isolates and differences in susceptibility between tulip and iris cultivars. At 18 degree C, but not at 9 degree C, isolates representing AG 2-2, AG 4, AG 5 and AG BI were pathogenic to bulbous crops. In addition to bare patch causing AG 2-t isolates, other anastomosis groups may cause disease in field grown tulips. For the development of optimal crop rotation schedules, the impact of bulb rot causing isolates under field conditions needs further study.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002775238

Download citation: RISBibTeXText

DOI: 10.1023/a:1008643311984

Related references

Genetic variability in pectic enzymes of Rhizoctonia solani isolates causing bare-patch disease of cereals. Journal of Phytopathology (Berlin) 141(3): 259-266, 1994

Characterization of cereal bare patch isolates of Rhizoctonia solani by random amplified polymorphic DNA analysis. Plant Pathology 44(5): 811-818, 1995

Identification of Rhizoctonia solani associated with field-grown tulips using its rDNA polymorphism and pectic zymograms. European Journal of Plant Pathology 103(7): 607-622, 1997

Evidence that each Rhizoctonia bare patch is dominated by an individual zymogram group (ZG) of Rhizoctonia solani AG-8. Australian Journal of Agricultural Research 44(6): 1175-1194, 1993

Characterization of Rhizoctonia solani isolates associated with patch diseases on turfgrass. Proceedings of the Oklahoma Academy of Science 84: 41-51, 2004

Characterization of thirteen Rhizoctonia solani isolates and pathogenicity of two isolates causing web blight on common bean in Puerto Rico. Annual report0(40): 91-92, 1997

Pectic zymogram characterisation used to demonstrate that each rhizoctonia bare patch in cereals is dominated by a single strain of rhizoctonia solani. Phytopathology 80(9): 890, 1990

Molecular Identification and Characterization of Rhizoctonia Solani Ag-3 ISOLATES CAUSING BLACK SCURF OF POTATO. Pakistan Journal of Botany 50(1): 415-420, 2018

Characterisation of Rhizoctonia solani anastomosis groups causing bottom rot in field-grown lettuce in Germany. European Journal of Plant Pathology 110(1): 53-62, 2004

Characterization of isolates of rhizoctonia solani from lima beans grown in new york state usa. Phytopathology 77(5): 748-751, 1987

Characterization by conventional techniques and PCR of Rhizoctonia solani isolates causing banded leaf sheath blight in maize. Plant Pathology 49(1): 108-118, 2000

Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal Biology 114(9): 691-701, 2010

Role of bacterial communities in the natural suppression of Rhizoctonia solani bare patch disease of wheat (Triticum aestivum L.). Applied and Environmental Microbiology 79(23): 7428-7438, 2013

Some characteristics of isolates of Rhizoctonia solani from patch of wheat and barley. Acta Mycologica 33(1): 109-121, 1998

Fungicide evaluation for the control of Rhizoctonia brown patch, 1981 Bentgrass (colonial) (Agrostis tenuis Astoria), Rhizoctonia brown patch; Rhizoctonia solani. Fungicide and nematicide tests results American Phytopathological Society7(37): 147, 1982