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Characterization of LSD binding to a serotonin-sensitive site in honeybee (Apis mellifera) brain



Characterization of LSD binding to a serotonin-sensitive site in honeybee (Apis mellifera) brain



Comparative Biochemistry and Physiology B, Biochemistry and Molecular Biology 112(2): 377-384



The binding of [3H]lysergic acid diethylamide ([3H]LSD) to a putative serotonin receptor site (S-site) in membrane preparations of honeybee brain was analyzed. All experiments were performed in the presence of 10 micromolar dopamine to inhibit [3H]LSD binding to an additional site, which is dopamine-sensitive (D-site). [3H]LSD binds reversibly to the S-site with a KD value of 0.89 nM. The maximal concentration of binding sites is 790 fmol/mg protein. Serotonin, lisuride, methysergide, 5-methoxytryptamine and tryptamine are the most potent displacers of S-site binding. The displacement efficacy of several other potential serotonin receptor agonists and antagonists was analyzed. Addition of the stable GTP analogue GTP S or high sodium concentrations reduced specific [3H]LSD binding to the putative serotonin receptor binding site.

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Accession: 002775280

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DOI: 10.1016/0305-0491(95)00098-4


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