+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a 17-kilodalton antigen of Bartonella henselae reactive with sera from patients with cat scratch disease



Characterization of a 17-kilodalton antigen of Bartonella henselae reactive with sera from patients with cat scratch disease



Journal of Clinical Microbiology 33(9): 2358-2365



A library of Bartonella (Rochalimaea) henselae DNA was constructed in the cloning vector lambda ZAPII and screened for expression of antigenic proteins by using a pool of sera from patients who had been diagnosed with cat scratch disease (CSD) and had antibodies to Bartonella spp., as determined by indirect fluorescent-antibody (IFA) assay. Ten immunoreactive phages were subcloned as recombinant plasmids by in vivo excision. All 10 recombinants expressed a protein of approximately 17 kDa when they were examined by immunoblot with the pool of human sera. Restriction endonuclease digestion of each recombinant plasmid indicated seven profiles, suggesting that cloning bias was not the reason for repeated isolation of clones expressing the 17-kDa antigen. The gene coding for the 17-kDa antigen was sequenced and shown to code for an open reading frame of 148 amino acids with a predicted molecular mass of 16,893 Da. The amino terminus of the deduced amino acid sequence was hydrophobic in nature and similar in size and composition to signal peptides found in gram-negative bacteria. The remainder of the deduced amino acid sequence was more hydrophilic and may represent surface-exposed epitopes. Further subcloning of the 17-kDa antigen as a biotinylated fusion protein in the expression vector PinPoint Xa-2 resulted in a 30-kDa protein that was highly reactive on immunoblots with individual serum samples from patients with CSD. The agreement between reactivity with the 30-kDa fusion protein on immunoblot analysis and the results obtained by IFA assay was 92% for IFA-positive sera and 88% for IFA-negative sera. The recombinant-expressed 17-kDa protein should be of value as an antigen for serologic diagnosis of CSD and Bartonella infections and warrants further study in attempts to develop a subunit vaccine to prevent long-term Bartonella infection in cats and the potential for further spread of these organisms to humans.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002775284

Download citation: RISBibTeXText

PMID: 7494028


Related references

The Barrosa breed. Veterinaria Tecnica 4(MAI/JUIN): 24-27, 2005

Improved cultural detection of Burkholderia cepacia from sputum in patients with cystic fibrosis. Journal of Clinical Pathology 54(10): 803-805, 2001

Evaluation of serological assays for diagnosis of onchocercosis. Scandinavian Journal of Infectious Diseases 29(1): 65-70, 1997

Bartonella henselae as the causative agent in cat-scratch disease: case report. Lijecnicki Vjesnik 123(1-2): 14-15, 2001

Bartonella henselae and Rickettsia felis Detected in Cat Fleas (Ctenocephalides felis) Derived from Eastern Austrian Cats. Vector Borne and Zoonotic Diseases 18(5): 282-284, 2018

Bartonella henselae infection in domestic cat and dog fleas. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 75(2): 133-136, 2001

The Utility of a Connecting Framework to Facilitate Understanding of and Reduce the Disparities in Hospice Care Experienced by Racial and Ethnic Minorities. Primary Psychiatry 15(10): 38-44, 2008

Isolation of Bacillus thuringiensis subsp. israelensis from disease field-collected larvae of the saturniid moth, Hylesia metabus, in French Guiana. FEMS microbiology letters Federation of European Microbiological Societies 107(2-3): 199-204, 1993

Bartonella henselae infection, the importance of images for diagnosis and follow-up. Revista Chilena de Infectologia 34(4): 410-412, 2017

Bartonella henselae in children with regional adenitis treated in a Peruvian national hospital, 2012. Revista Peruana de Medicina Experimental Y Salud Publica 31(2): 274-277, 2014

Predominance of Newcastle disease virus in the intestinal contents of chicks. Indian Veterinary Journal 74(7): 556-558, 1997

Transverse anisotropy of wood a function of gross anatomic structure. For. Prod. J, 9: 10, 350-9, 1959

Prevalence of antibodies to Anaplasma marginale in dairy herds in Bahia State, Brazil. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 50(3): 243-246, 1998

Low self-complementarity of cytochrome C messenger RNA, a structural and functional restriction for evolution at a molecular level. Journal theor Biol 542: 153-166, 1975

Natural Haemophilus influenzae Type b Capsular Polysaccharide Antibodies in 412 Infants and Children from West Africa (Burkina-Faso) and France: A Cross-Sectional Serosurvey. European Journal of Epidemiology 15(6): 577-582, 1999