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Characterization of a PCR fragment encoding 1-aminocyclopropane-1-carboxylate synthase in pepper (Capsicum annuum)



Characterization of a PCR fragment encoding 1-aminocyclopropane-1-carboxylate synthase in pepper (Capsicum annuum)



Journal of Plant Physiology 147(6): 661-664



Degenerate oligonucleotides corresponding to conserved domains found among diverse 1-aminocyclopropane-1-carboxylate (ACC) synthases (EC 4.4.1.14) were used with cDNA prepared from pepper fruit (pericarp) to isolate PCR fragments encoding ACC synthase in pepper. The clones isolated from green, turning and red pericarp were identical fragments of 1090 bp having a predicted 83% amino acid sequence similarity with the fruit-specific genes from tomato.

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Accession: 002775296

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DOI: 10.1016/s0176-1617(11)81475-6


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