+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a cDNA containing trinucleotide repeat sequences that is highly enriched in spermatogenic cells



Characterization of a cDNA containing trinucleotide repeat sequences that is highly enriched in spermatogenic cells



Molecular Reproduction and Development 46(4): 476-481



Trinucleotide repeat sequences have become of great interest due to their association with specific genetic disorders. Here we report the identification of a cDNA containing opa trinucleotide repeats from mouse testis, termed t-OPA. The opa repeat is contained within the longest open reading frame within the cDNA. Northern analysis demonstrated that four distinct t-OPA transcripts (1.6, 2.5, 3.6, 4.0 kilobases) are preferentially expressed in mouse and rat testis, with low expression in the pituitary, brain, and adrenal gland. Further, t-OPA RNAs were highly abundant in both pachytene spermatocytes and round spermatids and decreased in cytoplasts. Polysome profile analysis indicated that t-OPA mRNAs are translated in mouse testis with efficiencies similar to other transcripts expressed in late meiotic/early post-meiotic spermatogenic cells. These findings thus suggest a role for cell-specific mRNAs containing opa repeats during mouse spermatogenesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775307

Download citation: RISBibTeXText

PMID: 9094094

DOI: 10.1002/(sici)1098-2795(199704)46:4<476::aid-mrd5>3.0.co;2-l


Related references

RAPID cloning of expanded trinucleotide repeat sequences from genomic and cDNA samples. American Journal of Human Genetics 61(4 Suppl. ): A312, 1997

Microsatellite instability of dinucleotide tandem repeat sequences is higher than trinucleotide, tetranucleotide and pentanucleotide repeat sequences in prostate cancer. International Journal of Oncology 16(6): 1203-1209, 2000

Isolation, characterization and evolution of bovine trinucleotide and tetranucleotide microsatellite repeat sequences. Animal Genetics 27(Suppl. 2): 25, 1996

cDNA cloning of a human homologue of the Caenorhabditis elegans cell fate-determining gene mab-21: expression, chromosomal localization and analysis of a highly polymorphic (CAG)n trinucleotide repeat. Human Molecular Genetics 5(5): 607-616, 1996

Characterization of a highly polymorphic trinucleotide short tandem repeat within the human antithrombin gene. Thrombosis Research 74(3): 303-307, 1994

Characterization of trinucleotide- and tandem repeat-containing transcripts obtained from human spinal cord cDNA library by high-density filter hybridization. Dna and Cell Biology 19(5): 265-273, 2000

Detection of expanded trinucleotide repeat sequences in myotonic dystrophy patient cells. Molecular Biology of the Cell 7(Suppl. ): 300A, 1996

A small-insert bovine genomic library highly enriched for microsatellite repeat sequences. Mammalian Genome 6(10): 714-724, 1995

Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. Proceedings of the National Academy of Sciences of the United States of America 89(8): 3419-3423, 1992

Detection of trinucleotide repeat sequences in myotonic dystrophy patient cells using a single oligonucleotide probe. Molecular Biology of the Cell 5(Suppl. ): 337A, 1994

Localization of trinucleotide repeat sequences in myotonic dystrophy cells using a single fluorochrome-labeled PNA probe. Biotechniques 24(3): 472-476, 1998

Spatial distribution of expanded trinucleotide repeat sequences in Myotonic Dystrophy and Fragile x syndrome patient cells. American Journal of Human Genetics 59(4 Suppl. ): A49, 1996

CAG trinucleotide repeat sequences in exon 1 of the androgen receptor gene in prostatic tissue and carcinomas show limited polymorphism with a preferential repeat length of 19. Proceedings of the American Association for Cancer Research Annual Meeting 39: 629, 1998

Characterization of a cDNA highly enriched in CNS that shares sequence homology with the ICE/Ced-3 family. Society for Neuroscience Abstracts 22(1-3): 565, 1996

CDNA copies of the testis-specific lactate dehydrogenase (LDH-C) mRNA are present in spermatogenic cells in mice, but processed pseudogenes are not derived from mRNAs that are expressed in haploid and late meiotic spermatogenic cells. Mammalian Genome 10(1): 6-12, 1999