+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta



Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta



Applied and Environmental Microbiology 62(7): 2443-2448



A highly repeated DNA sequence from the genome of an arbuscular mycorrhizal fungus has been isolated and characterized. This 1,202-bp sequence (SC1) represents about 0.24% of the Scutellospora castanea genome, estimated to be 1 pg by flow cytometry. The sequence was shown to be a Scutellospora-specific probe in Southern blots and dot blot hybridizations. After complete sequencing of SC1, PCR primers were generated and used to amplify a 907-bp fragment from spores of S. castanea or from colonized Allium porrum roots. No amplification products were obtained with DNA from either spores or mycorrhizal root of other species of arbuscular mycorrhizal fungi. These primers were sufficiently specific for unequivocal detection of S. castanea in planta.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002775344

Download citation: RISBibTeXText

PMID: 8779584


Related references

DNA cloning and screening of a partial genomic library from an arbuscular mycorrhizal fungus, Scutellospora castanea. Mycorrhiza 4(6): 251-254, 1994

Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea. Applied and Environmental Microbiology 68(9): 4567-4573, 2002

Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology 8(6): 915-921, 1999

Detection of the arbuscular mycorrhizal fungus Scutellospora heterogama within roots using polyclonal antisera. Mycological Research 99(10): 1225-1232, 1995

Scutellospora castana, a newly described arbuscular mycorrhizal fungus. Cryptogamie Mycologie 14(4): 279-286, 1993

Construction of genomic phage libraries of the arbuscular mycorrhizal fungi Glomus mosseae and Scutellospora castanea and isolation of ribosomal RNA genes. Mycorrhiza 6(3): 167-173, 1996

Scutellospora persica , an arbuscular mycorrhizal fungus new to the mycota of Poland. Mycotaxon 65: 379-390, 1997

Scutellospora hawaiiensis: A new species of arbuscular mycorrhizal fungus from Hawaii. Mycologia 87(5): 678-683, 1995

Monoxenic culture of the arbuscular mycorrhizal fungus Scutellospora calospora and Ri-TDNA transformed carrot roots. New Zealand Plant Protection 59: 97-102, 2006

Heritable variation and mechanisms of inheritance of spore shape within a population of Scutellospora pellucida, an arbuscular mycorrhizal fungus. American Journal of Botany 86(9): 1209-1216, 1999

Ontogenetic comparisons of arbuscular mycorrhizal fungi Scutellospora heterogama and Scutellospora pellucida: Revision of taxonomic character concepts, species descriptions and phylogenetic hypotheses. Canadian Journal of Botany 72(1): 122-134, 1994

Cytoplasmic autofluorescence of an arbuscular mycorrhizal fungus Gigaspora gigantea and nondestructive fungal observations in planta. Mycologia 90(5): 921-926, 1998

RiPEIP1, a gene from the arbuscular mycorrhizal fungus Rhizophagus irregularis, is preferentially expressed in planta and may be involved in root colonization. Mycorrhiza 26(6): 609-621, 2016