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Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola

Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola

Mycologia 88(6): 996-1002

A polyphenol oxidase was demonstrated to be a component of the extracellular conidial mucilage of Colletotrichum graminicola. The enzyme was identified as a laccase based on its oxidation of syringaldazine in the absence of hydrogen peroxide and its absorption maximum at 593 nm, that is characteristic of blue, type 11 copper-containing laccases. The enzyme, purified by DEAE cellulose, Superose and MONO Q Fast Protein Liquid chromatographic procedures, had a molecular weight of 85 kD, a pH optimum of 6.0, and exhibited K-m and V-max values of 0.214 mmoles and 1.014 mmoles L-1 min-1, respectively, with syringaldazine as the substrate. The function of the enzyme is discussed relative to its potential role in fungal survival and detoxification of plant phenols.

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Accession: 002775345

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DOI: 10.2307/3761063

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