+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a new isolate of Ehrlichia platys (Order Rickettsiales) using electron microscopy and polymerase chain reaction



Characterization of a new isolate of Ehrlichia platys (Order Rickettsiales) using electron microscopy and polymerase chain reaction



Veterinary Parasitology 68(1/2): 1-10



A mixed-breed pup approximately 3 months old obtained in north central Oklahoma by the Laboratory Animal Resources Unit of Oklahoma State University presented with platelet inclusions. The dog developed severe thrombocytopenia (< 10,000 microliters-1) following the appearance of inclusions. Blood films were monitored daily and when about 75% of platelets had inclusions, samples were collected in EDTA and processed for electron microscopic (EM) studies and polymerase chain reaction (PCR). EM studies on glutaraldehyde-fixed buffy coat revealed rickettsia-like inclusions in numerous platelets. Serologic examination, using Ehrlichia platys antigen, showed high titre suggestive of E. platys infection. PCR primers derived from a highly variable region of the 16S rRNA gene sequence of E. platys were used to specifically amplify that region of the parasite's DNA. Sequencing of the PCR product obtained by general Ehrlichia primers showed one nucleotide difference from the published sequence for E. platys which suggests possible strain variation of this intracellular parasite. Our results indicate that PCR may be a useful tool in the diagnosis of E. platys infection and that, like other Ehrlichia spp., E. platys isolates may vary.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775371

Download citation: RISBibTeXText

PMID: 9066046

DOI: 10.1016/s0304-4017(96)01052-7


Related references

Two-step polymerase chain reaction for detection of Ehrlichia platys in canine infectious cyclic thrombocytopenia. Journal of the Chinese Society of Veterinary Science 22(1): 9-16, 1996

The prevalence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay. Annals of Parasitology 60(4): 267-272, 2014

Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. International Journal of Systematic & Evolutionary Microbiology 51(6): 2145-2165, 2001

Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. International Journal of Systematic and Evolutionary Microbiology 51(Pt 6): 2145-2165, 2001

Examining a tick population in order to detect Ehrlichia canis infection using polymerase chain reaction. Medycyna Weterynaryjna 62(5): 553-556, 2006

Infection with Ehrlichia canis and Anaplasma platys (Rickettsiales: Anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) from Argentina. Ticks and Tick-Borne Diseases 6(6): 724-729, 2015

Detection of anti-Ehrlichia antibodies and direct demonstration of Ehrlichia nucleic acid using the polymerase chain reaction (PCR) in patients in the Czech Republic. Klinicka Mikrobiologie a Infekcni Lekarstvi 10(1): 25-29, 2004

A real-time combined polymerase chain reaction assay for the rapid detection and differentiation of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Ehrlichia ewingii. Diagnostic Microbiology and Infectious Disease 53(4): 301-306, 2005

Detection and characterization of Rickettsia tsutsugamushi (Rickettsiales: Rickettsiaceae) in infected Leptotrombidium (Leptotrombidium) fletcheri chiggers (Acari: Trombiculidae) with the polymerase chain reaction. Journal of Medical Entomology 31(5): 691-699, 1994

Demonstration of Chlamydia trachomatis in inguinal lymphadenitis of lymphogranuloma venereum: a light microscopy, electron microscopy and polymerase chain reaction study. Modern Pathology 8(9): 924-929, 1995

Whipple's disease diagnosed using electron microscopy and polymerase chain reaction. Nihon Shokakibyo Gakkai Zasshi 113(11): 1894-1900, 2016

Mycoplasma pneumoniae pericarditis demonstrated by polymerase chain reaction and electron microscopy. Clinical Infectious Diseases 34(1): E16-E17, 2001

Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. Journal of Veterinary Diagnostic Investigation 15(6): 527-534, 2003