+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3'-end of a poly(A)+ transcript



Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3'-end of a poly(A)+ transcript



Gene 146(2): 245-250



We have carried out the molecular characterization of a highly repeated DNA element, called E12, from Trypanosoma cruzi, which has been found to be interspersed along its genome. The E12 element, repeated about 5.6 x 10(3) times, is found in most of the chromosomal bands of the parasite. Three subregions may be defined within the element on the basis of sequence similarities with other trypanosome genomic sequences. Northern blot analysis demonstrated that sequences of the E12 element are present in several polyadenylated RNA species of T. cruzi. The isolation and characterization of a cDNA clone, pSPFM55, which showed hybridization with the E12 probe, indicated that only one of the E12 subregions, E12A, is found in the cDNA and that it is located at the 3'-end providing the site of polyadenylation addition. The location and high degree of nucleotide conservation of E12A suggest a possible functional role of this sequence in gene expression.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775420

Download citation: RISBibTeXText

PMID: 8076826

DOI: 10.1016/0378-1119(94)90300-x


Related references

A novel reiterated family of transcribed oligo(A)-terminated, interspersed Dna elements in the genome of Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz 98(1): 129-133, 2003

A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Memorias do Instituto Oswaldo Cruz 98(1): 129-133, 2003

Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification. Molecular and Biochemical Parasitology 512: 271-280, 1992

Molecular characterization of the short interspersed repetitive element SIRE in the six discrete typing units (DTUs) of Trypanosoma cruzi. Experimental Parasitology 132(2): 144-150, 2012

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi. Parasitology 115: 563-570, 1997

Characterization of an interspersed repetitive Dna element in the genome of Trypanosoma cruzi. Parasitology 115(6): 563-570, 1997

A short interspersed repetitive element provides a new 3 acceptor site for trans-splicing in certain ribosomal P2 protein genes of Trypanosoma cruzi. Molecular and Biochemical Parasitology 64(2): 327-336, 1994

A short interspersed repetitive element provides a new 3' acceptor site for trans-splicing in certain ribosomal P2 beta protein genes of Trypanosoma cruzi. Molecular and Biochemical Parasitology 64(2): 327-336, 1994

The short interspersed repetitive element of Trypanosoma cruzi, SIRE, is part of VIPER, an unusual retroelement related to long terminal repeat retrotransposons. Proceedings of the National Academy of Sciences of the United States of America 97(5): 2128-2133, 2000

Characterization of Trypanosoma cruzi TcRjl locus and analysis of its transcript. Parasitology 129(Pt 3): 325-333, 2004

Characterization of a Trypanosoma cruzi poly(A)-binding protein and its genes. Molecular and Biochemical Parasitology 67(2): 301-312, 1994

Characterization of poly saccharides in crithidia fasciculata and trypanosoma cruzi. Federation Proceedings 35(7): 1733, 1976

CAMP receptor protein from Trypanosoma cruzi: purification and cloning of a short sequence of the corresponding cDNA. Biological Research 26(1-2): 257-265, 1993

Microorganism of the triatominae vectors of trypanosoma cruzi iii isolation and characterization of bacteria and fungi of the digestive tract of panstrongylus megistus negative and positive for trypanosoma cruzi. Memorias do Instituto Oswaldo Cruz 85(Suppl. 1): 114, 1990

Sequence of the 24S ribosomal RNA gene and characterization of a corresponding pseudogene from Trypanosoma cruzi. Molecular and Biochemical Parasitology 401: 35-42, 1990