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Characterization of bovine microsatellites by silver staining

Characterization of bovine microsatellites by silver staining

Journal of Animal Breeding and Genetics 112(5-6): 415-420

Silver staining was used to detect amplified microsatellite loci from the bovine genome. The standard silver-staining procedure was modified to allow more direct control in the development of signals. Microsatellites were analysed, to compare the silver-staining technique and the direct incorporation of a radioactive tracer. With silver staining, levels of sensitivity were achieved that are typical of those with radioisotopic detection. Also, silver staining is a cheaper process, is less hazardous to operators and the environment, and can be performed in laboratories without radioisotopic facilities. A significant advantage of silver staining is the speed of the process, producing results of a typing in 1 day.

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Accession: 002775523

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DOI: 10.1111/j.1439-0388.1995.tb00580.x

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