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Characterization of cis-prenyl transferase activity localised in a buoyant fraction of rubber particles from Ficus elastica latex

Characterization of cis-prenyl transferase activity localised in a buoyant fraction of rubber particles from Ficus elastica latex

Plant Physiology & Biochemistry 34(3): 377-384

We investigated rubber biosynthesis in Ficus elastica, a latex-producing plant species. Rubber particles were purified and enzyme activity was assayed by following the incorporation of radiolabel, from 14C-isopentenyl diphosphate, into newly synthesised rubber (cis-1,4-polyisoprene). We characterised the rubber particle-bound F. elastica cis-prenyl transferase (rubber transferase), and describe the association of the active enzyme with a buoyant fraction of latex rubber particles. Also, we provide experimental data that may explain how isolated F. elastica rubber particles were mistakenly believed to be enzymatically inert.

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