+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of class II A and B genes in a gynogenetic carp clone



Characterization of class II A and B genes in a gynogenetic carp clone



Immunogenetics 44(3): 192-202



A prerequisite for carrying out functional studies on major histocompatibility complex (Mhc) molecules of fish is the availability of genetically well-defined homozygous strains. Previously we have applied gynogenetic reproduction to generate isogenic carp, denoted clone A410. This clone has recently been demonstrated to express a single class I gene, Cyca-UA1(*)01, and in the present study two class II B and two class II A transcripts were obtained. The two class II B transcripts, Cyca-D(CB3)B and Cyca-D(CB4)B, as well as the class II A transcripts, Cyca-D(10A)A and Cyca-D(15A)A, appear to be bona fide class II transcripts, based on the presence of conserved protein characteristics of the inferred class II molecules. With the isolation of class II A sequences, representatives of all major classes of Mhc genes have been identified in the carp. To assess the relationship between the different class II genes, segregation studies, comparison of cDNA and intron 1 sequence data, and phylogenetic analyses were undertaken. These showed that the class II B transcripts, Cyca-D(CB3)B and Cyca-D(CB4)B, are derived from related, closely linked loci. In addition, these studies indicated that the previously described Cyca-DAB*01 and Cyca-DAB*02 are also closely linked, but that this linked pair segregates independently from the Cyca-D(CB3)B and Cyca-D(CB4)B loci. The class II A transcripts are most likely derived from separate loci and do not represent alleles, as they were found not to segregate in the individuals of the clone which was generated by meiogenetic gynogenesis.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002775573

Download citation: RISBibTeXText

PMID: 8662086


Related references

Cloning and characterization of full length cDNAs of two genes related to protein biosynthesis in gynogenetic gibel carp. Acta Hydrobiologica SinicaSep; 27(5): 512-520, 2003

Identification and expression analysis of genes involved in early ovary development in diploid gynogenetic hybrids of red crucian carp x common carp. Marine Biotechnology 12(2): 186-194, 2010

Fertilization biology of gynogenetic crucian carp (Carassius auratus gibelio), with a discussion on the reproductive modes of the naturally gynogenetic crucian carp. Acta Hydrobiologica Sinica 16(2): 97-100, 1992

Isozymes in androgenetic and gynogenetic white amur, gynogenetic carp, and carp-amur hybrids. Journal of Heredity 67(3): 129-134, 1976

The organization of hla class i genes sequencing of hla b8 hla bw62 genes a new hla c specific clone and identification of functional non hla a hla b hla c class i genes. Immunobiology 173(2-5): 273-274, 1986

Study of the gynogenetic progenies of the carassius auratus and cyprinus carpio hybrids as model objects for determining the possible mechanisms of clone formation in gynogenetic and hybridogenic fish. Voprosy Ikhtiologii 30(3): 425-431, 1990

Genetic diversity of artificial gynogenetic silver carp and RAPD analysis of incorporation of heterologous genetic materials in gynogenetic progeny. Acta Hydrobiologica Sinica 28(2): 180-185, 2004

Production of gynogenetic progeny from allotetraploid hybrids red crucian carp x common carp. Aquaculture e 14 236(1-4): 193-200, 2004

Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp x common carp. Science in China. Series C, Life Sciences 50(2): 186-193, 2007

Comparative cytological studies on the oocyte maturation in gynogenetic crucian carp and amphimictic red carp. Acta Hydrobiologica Sinica 15(2): 97-102, 1991

Studies on production of gynogenetic grass carp and crucian carp. Acta Scientiarum Naturalium Universitatis Normalis Hunanensis 14(2): 154-159, 1991

Production of hybrid androgenetic and gynogenetic grass carp and carp. Transactions of the American Fisheries Society 105(1): 10-16, 1976

An investigation of radiation-induced gynogenesis in the carp. 4. State of the gonads and reproductive ability of carp of gynogenetic origin. Genetika, USSR 15(9): 1643-1650, 1979

Induced tetraploid in the hybrid of natural gynogenetic female silver crucian carp and male common carp. Gall, G A E, Chen, H Genetics in aquaculture, IV: 316, 1993