+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of enzootic nasal tumor virus capsid antigen



Characterization of enzootic nasal tumor virus capsid antigen



Veterinary Microbiology 53(3-4): 261-269



The RT-PCR was carried out on tumor tissue from sheep with enzootic nasal tumor (ENT), using primers designed from conserved amino acid regions of related type D retroviruses. A 591 hp PCR fragment, corresponding to 90% of the capsid antigen was cloned, sequenced and expressed in E. coli. Alignment with ovine pulmonary carcinoma (OPC) virus showed 93% nucleotide and 96% amino acid homology. No amplification occurred when DNA from ovine fetal cell line was used as template. The recombinant protein, highly expressed in a prokaryotic system, reacted in immunoblot with mouse antiserum to Mason Pfizer monkey virus (MPMV) p27, as well as sera from OPC and ENT diseased animals. Preliminary application of this antigen in ELISA suggested its potential use to detect seropositive animals in infected flocks.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775624

Download citation: RISBibTeXText

PMID: 9008337

DOI: 10.1016/s0378-1135(96)01218-7


Related references

Glycohistochemical characterization of histologically normal nasal mucosa and enzootic nasal tumor of sheep. American Journal of Veterinary Research 73(8): 1128-1136, 2012

Mechanism of cell entry and transformation by enzootic nasal tumor virus. Journal of Virology 76(5): 2141-2149, 2002

Seroconversion of sheep experimentally infected with enzootic nasal tumor virus. Bmc Research Notes 9: 15, 2016

Improved enzootic nasal tumor virus pseudotype packaging cell lines reveal virus entry requirements in addition to the primary receptor Hyal2. Journal of Virology 79(1): 87-94, 2005

Enzootic nasal tumor virus envelope requires a very acidic pH for fusion activation and infection. Journal of Virology 82(18): 9023-9034, 2008

Evaluation of the abbot Architect(™) epstein-barr virus viral capsid antigen IgM, viral capsid antigen IgG and nuclear antigen IgG assays in a pediatric and adult population. Journal of Clinical Virology 81: 1-5, 2016

Complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep. Journal of Virology 73(5): 3986-3993, 1999

Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution. Journal of General Virology 84(Pt 8): 2245-2252, 2003

Truncation of the enzootic nasal tumor virus envelope protein cytoplasmic tail increases Env-mediated fusion and infectivity. Journal of General Virology 98(1): 108-120, 2017

Development of an ante-mortem diagnostic test for enzootic nasal tumor virus and detection of neutralizing antibodies in host serum. Journal of General Virology 95(Pt 8): 1843-1854, 2014

Characterization of the immunodominant cross-reacting epitope of visna maedi virus and caprine arthritis-encephalitis virus capsid antigen. Virus Research 61(2): 177-183, 1999

Full-length genome sequence analysis of enzootic nasal tumor virus reveals an unusually high degree of genetic stability. Virus Research 151(1): 74-87, 2010

Envelope proteins of Jaagsiekte sheep retrovirus and enzootic nasal tumor virus induce similar bronchioalveolar tumors in lungs of mice. Journal of Virology 80(18): 9322-9325, 2006

Signal transduction pathways utilized by enzootic nasal tumor virus (ENTV-1) envelope protein in transformation of rat epithelial cells resemble those used by jaagsiekte sheep retrovirus. Virus Genes 36(1): 147-155, 2008