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Characterization of epitopes on an 18 kDa piroplasm surface protein of Babesia equi



Characterization of epitopes on an 18 kDa piroplasm surface protein of Babesia equi



Journal of Protozoology Research 5(2): 47-57



Monoclonal antibodies (MAbs) were produced against Babesia equi. Three MAbs which reacted with an 18 000 MW surface membrane protein (p18) of B. equi in immunoblot analysis were used to characterize the epitopes of p18. All 3 MAbs recognized the same epitope on p18, as shown by competitive ELISA. Negative results in 2-site ELISA suggested the absence of repetitive epitopes on p18. Triton X-114 phase partitioning confirmed that the 18 000 MW antigen is an integral membrane protein of B. equi.

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