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Characterization of genetic biodiversity with Vitis vinifera L. Sangiovese and Colorino genotypes by AFLP and ISTR DNA marker technology

Characterization of genetic biodiversity with Vitis vinifera L. Sangiovese and Colorino genotypes by AFLP and ISTR DNA marker technology

Vitis 35(4): 183-188

A comparison between two recently developed, PCR-based DNA marker technologies (amplified fragment length polymorphism (AFLP) and inverse sequence-tagged repeat analysis (ISTR)) was carried out for 19 Vitis vinifera accessions, including 13 putative Sangiovese (SG)-related grapevines and 6 "coloured" ecotypes whose fruits are of importance for conferring intense red colour to the wine. Eight AFLP and 5 ISTR primer combinations generated 264 and 249 polymorphic markers, respectively.

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