+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of genetically transformed Saccharomyces cerevisiae baker's yeasts able to metabolize melibiose

Characterization of genetically transformed Saccharomyces cerevisiae baker's yeasts able to metabolize melibiose

Applied and Environmental Microbiology 61(6): 2113-2121

Three transformant (Mel+) Saccharomyces cerevisiae baker's yeast strains, CT-Mel, VS-Mel, and DADI-Mel, have been characterized. The strains, which originally lacked alpha-galactosidase activity (Mel-), had been transformed with a DNA fragment which possessed an ILV2-SMR1 allele of the ILV2 gene and a MEL1 gene. The three transformed strains showed growth rates similar to those of the untransformed controls in both minimal and semi-industrial (molasses) media. The alpha-galactosidase specific activity of strain CT-Mel was twice that of VS-Mel and DADI-Mel. The yield, YX/S (milligrams of protein per milligram of substrate), in minimal medium with raffinose as the carbon source was 2.5 times higher in the transformed strains than in the controls and was 1.5 times higher in CT-Mel than in VS-Mel and DADI-Mel. When molasses was used, YX/S (milligrams of protein per milliliter of culture) increased 8% when the transformed strains CT-Mel and DADI-Mel were used instead of the controls. Whereas no viable spores were recovered from either DADI-Mel or VS-Mel tetrads, genetic analysis carried out with CT-Mel indicated that the MEL1 gene has been integrated in two of three homologous loci. Analysis of the DNA content by flow cytometry indicated that strain CT-Mel was 3n, whereas VS-Mel was 2n and DADI-Mel was 1.5n. Electrophoretic karyotype and Southern blot analyses of the transformed strains showed that the MEL1 gene has been integrated in the same chromosomic band, probably chromosome XIII, in the three strains. Tetrads of CT-Mel strain segregated 4+:0-, 3+:1- and 2+:2-, for both positive MEL1 Southern blots and a-galactosidase activity, indicating, as expected, integration of the MEL1 gene in two of three homologous chromosomes.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 002775668

Download citation: RISBibTeXText

PMID: 7793932

Related references

Genomic stability of Saccharomyces cerevisiae baker's yeasts. Systematic and Applied Microbiology 22(3): 329-340, 1999

Chromosomal reorganization during meiosis of Saccharomyces cerevisiae baker's yeasts. Current Genetics 32(4): 247-259, 1997

Hybridization and selection of Saccharomyces cerevisiae strains from industrial baker's yeasts. Kasetsart Journal, Natural Sciences 38(2): 255-266, 2004

Construction of alpha galactosidase positive strains of industrial baker's saccharomyces cerevisiae yeasts. Journal of the American Society of Brewing Chemists 46(3): 67-71, 1988

Factors Which Affect the Frequency of Sporulation and Tetrad Formation in Saccharomyces cerevisiae Baker's Yeasts. Applied and Environmental Microbiology 61(4): 1677, 1995

Nutritive value of fodder yeasts. 3. Influence of some chemical and physiological factors on the utilisation of dried baker's yeast (Saccharomyces cerevisiae) by animals. Revista de Nutricion Animal 12(1): 17-26, 1974

Genetically different wine yeasts isolated from Austrian vine-growing regions influence wine aroma differently and contain putative hybrids between Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Fems Yeast Research 7(6): 953-965, 2007

Characterization of early deaths of non- Saccharomyces yeasts in mixed cultures with Saccharomyces cerevisiae. Archives of Microbiology 180(4): 257-263, 2003

Factors influencing the formations of a yeast head by brewing yeasts saccharomyces cerevisiae saccharomyces cerevisiae saccharomyces carlsbergensis. Journal of the Institute of Brewing 75(2): 200-204, 1969

Traditional Norwegian Kveik Are a Genetically Distinct Group of Domesticated Saccharomyces cerevisiae Brewing Yeasts. Frontiers in Microbiology 9: 2137, 2018

Isolation and characterization of paracrystalline arrays of the plasma membrane of baker's yeast Saccharomyces cerevisiae. European Journal of Cell Biology 24(2): 216-225, 1981

Partial purification and characterization of neutral trehalase from commercial baker's yeast, Saccharomyces cerevisiae. Journal of food biochemistry 24(6): 443-451, 2000

Molecular genetic diversity of the Saccharomyces yeasts in Taiwan: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Antonie van Leeuwenhoek 103(1): 217-228, 2013

Physical, functional and structural characterization of the cell wall fractions from baker's yeast Saccharomyces cerevisiae. Food Chemistry 194: 1149-1155, 2016

Purification and functional characterization of the pyruvate monocarboxylate carrier from baker's yeast mitochondria saccharomyces cerevisiae. Biochimica et Biophysica Acta 1079(1): 87-95, 1991