+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of leucine-leucine transport in leaf tissues



Characterization of leucine-leucine transport in leaf tissues



Journal of Experimental Botany 47 Spec No: 1223-1227



Uptake of the dipeptide [(3)H]Leu-Leu into leaf discs from mature broad bean (Vicia faba L.) was characterized. Uptake was maximal at pH 6.0 and appeared to be mediated by three systems with apparent K(m) values of 20 µM, 350 µM and 43 mM, respectively. Leu-Leu uptake was sensitive to N-ethylmaleimide, p-chloromercuribenzenesulphonic acid, diethylpyrocarbonate, and carbonyl-cyanide-m-chlorophenylhydrazone. Nitrate did not compete with peptide uptake, although the peptide transporter and the nitrate transporter have been reported to be homologous. The ability of leaf tissues to take up peptides strongly decreased with leaf age, and the phloem export of peptides as measured by exudation experiments was very low. It is concluded that the leaf tissues contain a peptide transporter that may take up some peptides with a high affinity, but that this transporter is not involved in the long-distance transport of nitrogen under the form of di- or tri-peptides.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002775760

Download citation: RISBibTeXText

PMID: 21245253

DOI: 10.1093/jxb/47.special_issue.1223


Related references

Increased serum leucine, leucine-37 levels in psoriasis: positive and negative feedback loops of leucine, leucine-37 and pro- or anti-inflammatory cytokines. Human Immunology 71(12): 1161-1171, 2010

Pathway for iso leucine formation from pyruvate by leucine biosynthetic enzymes in leucine accumulating iso leucine revertants of serratia marcescens. Journal of Biochemistry 82(1): 95-104, 1977

Semi synthesis and biological properties of the b 24 leucine b 25 leucine and b 24 leucine b 25 leucine analogs of human insulin. Experientia 37(8): 811-813, 1981

Semisynthesis and biological properties of the [B24-leucine]-, [B25-leucine[- and [B24-leucine, B25-leucine]-analogues of human insulin. Experientia 37(8): 811-813, 1981

The effect of the administration of morphine metab to rats on the transport of d leucine and l leucine out of the cerebral spinal fluid abstract membrane transport level. Federation Proceedings 28(2): 735, 1969

Effect of D(+)-leucine on the hydrolysis of glycil-L-leucine in normal and neoplastic tissues. Nature 170(4332): 804, 1952

Leucine synthesis in chickens and leucine 2 3 aminomutase activity in tissues. Poultry Science 64(Suppl. 1): 196, 1985

Leucine transport mutants and the regulation of leucine transport in escherichia coli. Federation Proceedings 36(3): 827, 1977

Purification of a leucine specific binding protein and evidence for a 2nd leucine transport system. Federation Proceedings 29(2): 341, 1970

The effects of l phenyl alanine and d leucine on the transport of l leucine across the blood testis barrier in the rat. Journal of Physiology (Cambridge) 326: 17P-18P, 1982

Regulation of leucine iso leucine and valine transport activity in escherichia coli. Federation Proceedings 34(3): 492, 1975

Differential transport properties of D-leucine and L-leucine in the archaeon, Halobacterium salinarum. Canadian Journal of Microbiology 46(4): 376-382, 2000

Toxicity of leucine-containing peptides in Escherichia coli caused by circumvention of leucine transport regulation. Journal of Bacteriology 146(2): 676-683, 1981

Mechanisms of inhibition of glycylglycine transport by glycyl-L-leucine and L-leucine in guinea-pig small intestine. Biochimica et Biophysica Acta 687(2): 170-178, 1982

Subclinical infection with the nematode Trichostrongylus colubriformis increases gastrointestinal tract leucine metabolism and reduces availability of leucine for other tissues. Journal of Animal Science 78(2): 380-390, 2000