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Characterization of phenoloxidase from the haemolymph of Spodoptera litura larvae



Characterization of phenoloxidase from the haemolymph of Spodoptera litura larvae



Korean Journal of Entomology 27(2): 143-152



For studies of non- self recognition in insects, we investigated the phenoloxidase (PO) system from the larval haemolymph of Spodoptera litura. PO is present in haemolymph and detected as an intense band with high molecular weight by electrophoresis. The enzyme catalysed the oxidation of other di-phenols, besides dopa. The enzyme activity was stable up to 70 degree C and showed a pH optimum 8.0 in the reaction mixture containing L-dopa. The Km value for PO was 1.5 nM at pH 7.4 and pI value was 8.2. PTU, the specific inhibitor for dopamin beta-hydroxylase, strongly inhibited the PO activity: half maximal inhibition by PTU occurred at 12 mu-M, suggesting that Cu-2+ is important for the activity of PO. While PO can be activated by organic solvents, heat treatment or other environmental effect, suggesting in vivo conversion system that inactive PO might be changed to active PO by external stimulus.

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