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Cloning and expression of cryIVD gene of insecticidal crystal protein of Bacillus thuringiensis in the acrystalliferous strain


Cloning and expression of cryIVD gene of insecticidal crystal protein of Bacillus thuringiensis in the acrystalliferous strain



Entomologia Sinica 2(3): 239-246



DOI: 10.1111/j.1744-7917.1995.tb00045.x

The plasmid pGEM1, carrying cryIVD, cytA and 20-kDa protein genes, was extracted and digested with the fragment HindIII/BAMHI. A 5.4 kb fragment containing cryIVD and 20-kDa protein gene was purified and ligated to an Escherichia coli TG1. The recombinant plasmid was analysed by restriction mapping and Southern blotting to determine the correct insert. Then the recombinant was transferred into a crystalliferous mutant B. thuringiensis subsp. israelensis (Bti IPS 78/11) by electroporation.

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Accession: 002780025

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