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Cloning, production, characterization and IgE cross-reactivity of different manganese superoxide dismutases in individuals sensitized to Aspergillus fumigatus

Cloning, production, characterization and IgE cross-reactivity of different manganese superoxide dismutases in individuals sensitized to Aspergillus fumigatus

International Archives of Allergy and Immunology 113(1-3): 213-215

ISSN/ISBN: 1018-2438

PMID: 9130526

DOI: 10.1159/000237550

Background: Manganese superoxide dismutase (MnSOD) from Aspergillus fumigatus has been demonstrated to be an allergen showing a high degree of homology with phylogenetically distant MnSODs. We describe cloning, production and characterization of MnSODs from different species. Methods: MnSODs were cloned by PCR, expressed as inclusion body protein in Escherichia coli, purified by Ni-2+-chelate affinity chromatography and characterized. Results: The MnSODs from A. fumigatus, man, yeast, Drosophila melanogaster and E. coli show about 50% identity and 70% similarity on the primary structure. All proteins were produced at a high level in E. coli and refolded to achieve enzymatic activity. The proteins were able to bind IgE from sera of individuals sensitized to the A. fumigatus MnSOD. Conclusions: MnSOD represents a novel pan-allergen restricted to individuals sensitized to A. fumigatus.

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Accession: 002780410

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