EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Cloning, production, characterization and IgE cross-reactivity of different manganese superoxide dismutases in individuals sensitized to Aspergillus fumigatus


Cloning, production, characterization and IgE cross-reactivity of different manganese superoxide dismutases in individuals sensitized to Aspergillus fumigatus



International Archives of Allergy and Immunology 113(1-3): 213-215



ISSN/ISBN: 1018-2438

PMID: 9130526

DOI: 10.1159/000237550

Background: Manganese superoxide dismutase (MnSOD) from Aspergillus fumigatus has been demonstrated to be an allergen showing a high degree of homology with phylogenetically distant MnSODs. We describe cloning, production and characterization of MnSODs from different species. Methods: MnSODs were cloned by PCR, expressed as inclusion body protein in Escherichia coli, purified by Ni-2+-chelate affinity chromatography and characterized. Results: The MnSODs from A. fumigatus, man, yeast, Drosophila melanogaster and E. coli show about 50% identity and 70% similarity on the primary structure. All proteins were produced at a high level in E. coli and refolded to achieve enzymatic activity. The proteins were able to bind IgE from sera of individuals sensitized to the A. fumigatus MnSOD. Conclusions: MnSOD represents a novel pan-allergen restricted to individuals sensitized to A. fumigatus.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002780410

Download citation: RISBibTeXText

Related references

The Cu,Zn superoxide dismutases of Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, and Aspergillus terreus: purification and biochemical comparison with the Aspergillus fumigatus Cu,Zn superoxide dismutase. Infection and Immunity 64(8): 3326-3332, 1996

Immunological and structural analysis of IgE-mediated cross-reactivity between manganese superoxide dismutases. International Archives of Allergy and Immunology 128(4): 292-303, 2002

Cloning and characterization of rice manganese superoxide dismutases. Taiwania 42(1): 53-62, 1997

Cloning, expression, and characterization of two manganese superoxide dismutases from Caenorhabditis elegans. Journal of Biological Chemistry 272(45): 28652-28659, 1997

Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus × giganteus. Plant Cell Reports 34(12): 2137-2149, 2015

Cloning, expression, characterization, and computational approach for cross-reactivity prediction of manganese superoxide dismutase allergen from pistachio nut. Allergology International: Official Journal of the Japanese Society of Allergology 59(3): 295-304, 2010

Cloning and characterization of genes encoding the iron and manganese superoxide dismutases in pseudomonas aeruginosa. Abstracts of the General Meeting of the American Society for Microbiology 92: 105, 1992

Isolation and characterization of a cDNA for mitochondrial manganese superoxide dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutases. Biochimica et Biophysica Acta 951(1): 61-70, 1988

Characterization of antigens from Aspergillus fumigatus. V. Reactivity in immunodiffusion tests with serums from patients with aspergillosis caused by Aspergillus flavus, A. niger, and A fumigatus. American Review of Respiratory Disease 122(4): 647-650, 1980

Skin test reactivity to 2 recombinant Aspergillus fumigatus allergens in A fumigatus-sensitized asthmatic subjects allows diagnostic separation of allergic bronchopulmonary aspergillosis from fungal sensitization. Journal of Allergy and Clinical Immunology 104(3 Pt 1): 601-607, 1999

Characterization of superoxide dismutases in cardiac progenitor cells demonstrates a critical role for manganese superoxide dismutase. Stem Cells and Development 21(17): 3136-3146, 2012

Intracellular superoxide level controlled by manganese superoxide dismutases affects trichothecene production in Fusarium graminearum. Fems Microbiology Letters 364(21), 2017

Production and characterization of recombinant Aspergillus fumigatus Cu,Zn superoxide dismutase and its recognition by immune human sera. Journal of Clinical Microbiology 38(2): 558-562, 2000

EPR and ligand field studies of iron superoxide dismutases and iron-substituted manganese superoxide dismutases: relationships between electronic structure of the active site and activity. Inorganic Chemistry 39(12): 2666-2675, 2000

Cloning, overexpression, and characterization of a highly active endoinulinase gene from Aspergillus fumigatus Cl1 for production of inulo-oligosaccharides. Applied Biochemistry and Biotechnology 175(2): 1153-1167, 2015