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Comparative trials with anther culture derived doubled haploids and single seed descent lines in wheat



Comparative trials with anther culture derived doubled haploids and single seed descent lines in wheat



Cereal Research Communications 23(3): 267-273



The effect of the anther culture method on agronomic and quality characters has been investigated in 6 spring wheat crosses. Doubled haploids have been compared with single seed descent derived lines from the same crosses in one- or two-year trials. Maturity, plant height, resistance to mildew and yellow rust, biological yield, grain yield, Failing Number, seed protein and SDS sedimentation have been studied. Analyses of variance detected highly significant genetic variation among genotypes derived by either method in all cross/character combinations. The DHs were inferior to their SSD counterparts in height and yield characters in four of the crosses. Maturity, resistance to mildew, Falling number and seed protein (%) were unaffected. Resistance to yellow rust was reduced in four crosses and SDS showed no consistent pattern. The results indicate that negative effects of anther culture occur, but their presence and magnitude depend on the cross and character studied. It is suggested that the conflicting reports of anther culture effects on agronomic characters in wheat may be a reflection of the different genotypes studied.

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