EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Cytoplasmic-nuclear male sterility in pearl millet: comparative RFLP and transcript analyses of isonuclear male-sterile lines



Cytoplasmic-nuclear male sterility in pearl millet: comparative RFLP and transcript analyses of isonuclear male-sterile lines



Theoretical and Applied Genetics 95(5/6): 961-968



The identification of diagnostic cytoplasmic molecular markers is of prime interest to pearl millet breeders wishing to identify sources of cytoplasmic-nuclear male sterility (CMS) which can be used as an alternative to the single source currently used in the production of F1 hybrid seed. Here, we report the classification of five pearl millet CMS sources based on RFLP analysis of isonuclear lines carried out using mitochondrial gene-specific DNA probes in combination with eight restriction endonucleases. On the basis of RFLP data, the five CMS cytoplasms can be distinguished from each other and from the isonuclear fertile cytoplasm. In addition, based on cox1, cox3, atp6 and atp9 polymorphisms, these lines can be classified into two major groups: one corresponds to A5, Aegp, Av and A1 cytoplasms, and the other consists of the A4 cytoplasm. Our results suggest that a rearrangement involving the cox1 gene might be related to CMS in the first group (A5, Aegp, Av and A1), whereas a rearrangement within the atp6/cox3 cluster region might be related to CMS in the second group (A4).

(PDF emailed within 0-6 h: $19.90)

Accession: 002793214

Download citation: RISBibTeXText

DOI: 10.1007/s001220050648



Related references

Assessment of cytoplasmic differences of near-isonuclear male-sterile lines in pearl millet. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 87(1-2): 106-112, 1993

Cytoplasmic differentiation using near-isonuclear polycytoplasmic male sterile lines in pearl millet. Euphytica 67(1-2): 127-134, 1993

Esterase isozyme patterns in three isonuclear cytoplasmic male sterile lines of pearl millet. Crop Improvement 20(1): 81-87, 1993

Polyamine levels in anthers of poly cytoplasmic isonuclear male sterile lines of pearl millet. Botanical Bulletin of Academia Sinica (Taipei) 33(1): 97-100, 1992

Studies on the breakdown of male sterility and other related aspects in certain cytoplasmic male-sterile lines of pearl millet (Pennisetum typhoides Stapf and Hubb). Andhra Agricultural Journal 17(6): 173-180, 1972

RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 88(3-4): 441-448, 1994

Pb 310a 2 and pb 406a 3 isonuclear male sterile lines of pearl millet. Journal of Research Punjab Agricultural University 27(2): 379, 1990

Performance of pearl millet isonuclear hybrids involving different cytoplasmic male-sterility systems. Plant Breeding 115(2): 140-142, 1996

Studies on the breakdown of male sterility in some male sterile lines of pearl millet pennisetum typhoides under conditions of arid zone. Annals of Arid Zone 16(4): 427-432, 1977

Male fertility restoration and attributes of the A4 cytoplasmic-nuclear male sterile system for grain production in pearl millet. International Sorghum and Millets Newsletter 35: 64, 1994

A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA. Genome 37(3): 482-486, 1994

Fertile sterility maintainer mutants in cytoplasmic male sterile pearl millet. Crop Science 17(4): 635-637, 1977

Phosphatase and ATPase activities in isonuclear lines of cytoplasmic male-sterile and male-fertile petunia. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 86(1): 49-53, 1993

Differences in amino acid transport in isonuclear lines of cytoplasmic male-sterile and male-fertile petunia. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 84(1-2): 92-96, 1992

A new cytoplasmic-nuclear male sterility system in pearl millet. Plant Breeding 114(5): 445-447, 1995