DNA fingerprinting of bluegill sunfish (Lepomis macrochirus) using (GT) n microsatellites and its potential for assessment of mating success

Colbourne, J.K.nneth.; Neff, B.D.; Wright, J.M.; Gross, M.R.

Canadian Journal of Fisheries and Aquatic Sciences 53(2): 342-349


ISSN/ISBN: 0706-652X
DOI: 10.1139/f95-179
Accession: 002793458

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60 (GT) repeat microsatellite sequences from the bluegill sunfish genome were cloned and characterized. The frequency distribution, lengths, and the proportions of the repeat classes were compared with previous studies on other teleosts, mammals and invertebrates. Bluegill microsatellites were similar to those of other fish species. Primers for polymerase chain reaction amplification were designed for 17 bluegill microsatellite loci. The allele frequencies, degree of polymorphism and heterozygosity were estimated using bluegill from Lake Opinicon, Ontario. Seven loci were potentially highly informative for fingerprinting studies of parentage. A preliminary paternity analysis using 2 of the loci on brood from a natural nest revealed their potential use for estimating the relative success of the 2 reproductive life histories of bluegill males (i.e. older males that show parental care for the nest, and younger males that successfully compete for fertilization of the nest but do not participate in parental care (cuckolders)). Cross-amplification using these microsatellite markers on related fish species showed some degree of locus conservation among taxa, potentially allowing DNA fingerprinting of a variety of centrarchids.