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Detection of tomato spotted wilt virus using silica capture reverse transcription polymerase chain reaction (SC-RT-PCR) and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR)



Detection of tomato spotted wilt virus using silica capture reverse transcription polymerase chain reaction (SC-RT-PCR) and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR)



Phytopathologia Polonica ( 22): 35-40



Diagnostic methods SC-RT-PCR and IC-RT-PCR, based on polymerase chain reaction and 2 different techniques of viral RNA isolation, were used for tomato spotted wilt tospovirus (TSWV) detection. A specific PCR product of expected size was obtained for samples of infected plant sap, but not for negative control.

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