+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of turkey rhinotracheitis virus from chickens with swollen head syndrome by reverse transcriptase-polymerase chain reaction (RT-PCR)



Detection of turkey rhinotracheitis virus from chickens with swollen head syndrome by reverse transcriptase-polymerase chain reaction (RT-PCR)



Journal of Veterinary Medical Science 58(4): 359-361



For this sensitive and specific, reverse transcriptase-polymerase chain reaction (RT-PCR) method, for detection of turkey rhinotracheitis virus (TRTV), 2 sets of primers were designed from the F protein gene sequence of TRTV 3B strain. Sensitivity of detection by nested PCR with the primers corresponded to 0.4 TCID50. Applying this method to a field case of swollen head syndrome (SHS), TRTV could be detected directly from chicken trachea and turbinates.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002797338

Download citation: RISBibTeXText

PMID: 8741270


Related references

Detection of Turkey Rhinotracheitis Virus from Chickens with Swollen Head Syndrome by Reverse Transcriptase-Polymerase Chain Reaction(Rt-Pcr). The Journal of Veterinary Medical Science 58(4): 359-361, 1996

Pelacakan kasus flu burung pada ayam dengan reverse transcriptase polymerase chain reaction* detection of avian influenza in chickens by reverse transcriptase polymerase chain reaction. 2013

Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum (vol 56, pg 269, 2006). Diagnostic Microbiology and Infectious Disease 57(3): 353, 2007

Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum. Diagnostic Microbiology and Infectious Disease 56(3): 269-274, 2006

Detection of human immunodeficiency virus type 2 by real time reverse transcriptase polymerase chain reaction and nested reverse transcriptase polymerase chain reaction. 2007

Comparison of virus isolation, immunohistochemistry, and reverse transcriptase-polymerase chain reaction procedures for detection of turkey coronavirus. Avian diseasest 44(3): 624-631, 2000

Attempts to reproduce swollen head syndrome in specific pathogen-free chickens by inoculating with Escherichia coli and/or turkey rhinotracheitis virus. Avian Pathology 27(1): 21-27, 1998

Detection of turkey rhinotracheitis virus in turkeys using the polymerase chain reaction. Avian Pathology 22(4): 771-783, 1993

Presumptive detection of yellow head virus by reverse transcriptase-polymerase chain reaction and dot-blot hybridization in Litopenaeus vannamei and L. stylirostris cultured on the Northwest coast of Mexico. Journal of Fish Diseases 29(12): 717-726, 2006

Swollen head syndrome is not associated with turkey rhinotracheitis virus. Veterinary Record 132(2): 41-42, 1993

Detection of rabies virus by reverse transcriptase-polymerase chain reaction. Abstracts of the General Meeting of the American Society for Microbiology 101: 266, 2001

Detection of bluetongue virus (BTV) by reverse transcriptase polymerase chain reaction (RT-PCR). Israel Journal of Veterinary Medicine 52(1): 32-33, 1997

Detection and differentiation of European and American genotypes of porcine reproductive and respiratory syndrome virus (PRRSV) by modified one tube reverse transcriptase nested polymerase chain reaction (RT-PCR). Veterinarija ir Zootechnika (21): 29-36, 2003

Detection of measles virus by reverse-transcriptase polymerase chain reaction in a placenta. Journal of Maternal-Fetal and Neonatal Medicine 23(8): 935-937, 2010

Detection of avian pneumovirus infection in chickens and turkeys by indirect fluorescent antibody test and reverse transcriptase-polymerase chain reaction. Ankara Universitesi Veteriner Fakultesi Dergisi 52(3): 179-184, 2005