+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of PCR-based techniques to identify porcine transmissible gastroenteritis coronavirus isolates



Development of PCR-based techniques to identify porcine transmissible gastroenteritis coronavirus isolates



Canadian Journal of Veterinary Research 61(3): 167-172



Sixteen isolates of transmissible gastroenteritis virus and one isolate of porcine respiratory coronavirus were characterized using RT-PCR amplification of 4 antigenic subsites in the site A epitope on the TGEV spike gene. The PCR products were digested with restriction enzymes Sau3AI and SspI and the sizes of the fragments were determined. Three different digestion patterns were observed with each enzyme. The recognition site for Sau3AI was missing in 1 isolate, was present in 13 isolates and 3 isolates had 2 sites. PCR-products with a single site had 3 different fragment sizes and the other isolates produced 2 fragments with different sizes. The SspI recognition site was not present in 5 isolates and 12 isolates had a single site that produced 2 fragments of different sizes. Based on the restriction fragment sizes, the 17 isolates were separated into 7 groups. Direct sequencing of the 455 hp nested set fragments demonstrated greater than 96% sequence homology among the 16 isolates and 100% homology in the 4 antigenic subsites in the conserved site A epitope. The groups are discussed in relation to their sequence homology and virulence. In vitro procedures have been developed to identify several porcine enteric coronavirus isolates at the strain level.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002799176

Download citation: RISBibTeXText

PMID: 9242995


Related references

Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd. Archives of Virology 145(6): 1133-1147, 2000

Use of nonradioactive cDNA probes to differentiate porcine respiratory coronavirus and transmissible gastroenteritis virus isolates. Journal of Veterinary Diagnostic Investigation 8(2): 241-244, 1996

Cellular immune responses of pigs after primary inoculation with porcine respiratory coronavirus or transmissible gastroenteritis virus and challenge with transmissible gastroenteritis virus. Veterinary Immunology & Immunopathology 48(1-2): 35-54, 1995

Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus. American journal of veterinary research 54(2): 254-259, 1993

Field validation of a commercial blocking ELISA to differentiate antibody to transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus and to identify TGEV-infected swine herds. Journal of Veterinary Diagnostic Investigation 14(2): 97-105, 2002

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus. Journal of General Virology 74: 1795-1804, 1993

Porcine transmissible gastroenteritis (TGE) diagnosis and its differentiation from porcine respiratory coronavirus (PRCV). Veterinaria Italiana 33(23): 52-55, 1997

Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits. Msphere 4(2):, 2019

Sequence analysis of the leader rna of two porcine coronaviruses transmissible gastroenteritis virus and porcine respiratory coronavirus. Virus Genes 4(4): 289-302, 1990

Identification of phenanthroindolizines and phenanthroquinolizidines as novel potent anti-coronaviral agents for porcine enteropathogenic coronavirus transmissible gastroenteritis virus and human severe acute respiratory syndrome coronavirus. Antiviral Research 88(2): 160-168, 2010

ORF3a deletion in field strains of porcine-transmissible gastroenteritis virus in China: A hint of association with porcine respiratory coronavirus. Transboundary and Emerging Diseases 64(3): 698-702, 2017

Differentiation of porcine coronavirus from transmissible gastroenteritis virus. Veterinary Record 122(4): 86-87, 1988

Expression of the porcine transmissible gastroenteritis coronavirus M protein. Advances in Experimental Medicine and Biology 276: 121-126, 1990

Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity. Journal of Virology 70(8): 5634-5637, 1996

Cultivation techniques for animal coronaviruses: Emphasis on feline infectious peritonitis virus, canine coronavirus, transmissible gastroenteritis virus, and porcine hemagglutinating encephalomyelitis virus. Methods in Cell Science 11(2): 95-100, 1988