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Diagnosis of citrus tristeza virus with different monoclonal antibodies






Fitopatologia Brasileira 22(2): 191-194

Diagnosis of citrus tristeza virus with different monoclonal antibodies

Citrus tristeza virus was evaluated by biological indexing, ELISA, and western blot using monoclonal and polyclonal antibodies in citrus species and varieties. Severe strains were detected in mandarins originated by nucellar embriony. In all sweet orange varieties, with the exception of 'Hamlin', only mild strains were detected. Lower virus concentrations were found in usually tolerant species, such as the mandarin varieties of 'Poncan' and 'Willowleaf'. The virus concentration in a tree was higher on the western side. The absorption relationship (R-MU/MCA13) as determined by ELISA with the universal monoclonal antibodies 3DF1 + 3CA5 and MCA13, was used as indirect measurement to evaluate complex or mixtures of strains. Low values of R-A40-5, together with high values of A-405 as determined by MCA13 can be an indication that a great part of the virus is recognized by that monoclonal antibody, and vice-versa.


Accession: 002800770



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