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Diagnosis of citrus tristeza virus with different monoclonal antibodies






Fitopatologia Brasileira 22(2): 191-194

Diagnosis of citrus tristeza virus with different monoclonal antibodies

Citrus tristeza virus was evaluated by biological indexing, ELISA, and western blot using monoclonal and polyclonal antibodies in citrus species and varieties. Severe strains were detected in mandarins originated by nucellar embriony. In all sweet orange varieties, with the exception of 'Hamlin', only mild strains were detected. Lower virus concentrations were found in usually tolerant species, such as the mandarin varieties of 'Poncan' and 'Willowleaf'. The virus concentration in a tree was higher on the western side. The absorption relationship (R-MU/MCA13) as determined by ELISA with the universal monoclonal antibodies 3DF1 + 3CA5 and MCA13, was used as indirect measurement to evaluate complex or mixtures of strains. Low values of R-A40-5, together with high values of A-405 as determined by MCA13 can be an indication that a great part of the virus is recognized by that monoclonal antibody, and vice-versa.


Accession: 002800770



Related references

Vela C.; Cambra M.; Cortes E.; Moreno P.; Miguet J.G.; Perez D.S.n Roman C.; Sanz A., 1986: Production and characterization of monoclonal antibodies specific for citrus tristeza virus and their use for diagnosis. Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were cha...

Peroni, Lís.Antonio.; Lorencini, Márcio.; dos Reis, Jé.Raimundo.Ribeiro.; Machado, M.Antonio.; Stach-Machado, D.Ruth., 2009: Differential diagnosis of Brazilian strains of Citrus tristeza virus by epitope mapping of coat protein using monoclonal antibodies. Citrus tristeza virus (CTV) is one of the most important citrus pathogen, and among Brazilian CTV strains, the genotype Capão Bonito (CB) is the most harmful. Therefore, the coat protein (CP) gene were cloned and expressed as recombinant protein...

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Cambra M.; Garnsey S.M.; Permar T.A.; Henderson C.T.; Gumpf D.; Vela C., 1990: Detection of citrus tristeza virus ctv with a mixture of monoclonal antibodies. Phytopathology 80(10): 1034

Permar, T.; Garnsey, S., 1992: Monoclonal antibodies which discriminate between strains of citrus tristeza virus. Monoclonal cell lines producing antibodies to severe citrus tristeza virus have been developed. The antibodies react with crude plant extracts infected with severe isolates, but not with crude plant extracts infected with mild isolates or healthy...

Wang, C-Xia.; Wang, G-Ping.; Hong, N.; Jiang, B.; Liu, H.; Wu, K-Wei., 2006: Production of polyclonal and monoclonal antibodies against citrus tristeza virus and their efficiency for the detection of the virus. Citrus tristeza virus (CTV) was purified from a citrus sample by a modified protocol, and the yield was about 1 mg from 100 g citrus tissues. Polyclonal antibody was prepared by immunizing rabbits with the purified CTV preparation with a titer 1:2...

Gumpf D.J.; Zheng G Y.; Moreno P.; Diaz J.M., 1987: Production and evaluation of specific monoclonal antibodies to citrus tristeza virus strains. Hybridomas were prepared by the fusion of mouse myeloma cells, cell line P3-X63-Ag8.653, and spleen cells from BALB/C mice immunized with partially clarified and concentration sap from citrus tristeza virus (CTV) infected citron. A CTV strain, T51...

Cambra, M.; Moreno, P.; Martinez, M.E.; Lavina, A.; Perez de San Roman, C.; Cortes, E.; Miguet, J.G.; Sanz, A.; Vela, C., 1988: Comparison of monoclonal and polyclonal antibodies for the detection of citrus tristeza virus (CTV) by ELISA-DAS. Of various monoclonal antibodies (MA) specific to citrus tristeza closterovirus (CTV) one, 3DF1, was used to detect 23 isolates of the virus from different parts of Spain and 3 from other countries. The sensitivity of this MA was compared with tha...

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