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Dietary iron and fat affect nonheme iron absorption, iron status, and enterocyte aconitase activity and iron concentration in rats

Dietary iron and fat affect nonheme iron absorption, iron status, and enterocyte aconitase activity and iron concentration in rats

Nutrition Research 16(6): 977-989

The cytosolic iron regulatory protein 1 (IRP1) exhibits aconitase (c-acon) activity when it is iron loaded. Enterocyte c-acon activity may be an index of 1RP1 activity and important in the regulation of iron metabolism and absorption. Because stearic acid (SA) can enhance iron absorption the relationship between absorption and enterocyte c-acon activity in iron-deficient rats fed different types and amounts of dietary fat was determined. Rats (8/group) were fed diets varying in iron (10 or 35 mu-g/g), fat type (SA, safflower oil (Saff), cocoa butter (CB), or beef tallow (BT)) and fat amount (7 or 15%). Rats killed for baseline values were iron-deficient with low enterocyte c-acon activity. Absorption was assessed by 59Fe retention. After 4 wk, DELTA Hb and c-acon activity were less (p lt 0.001) and iron absorption was greater (p lt 0.001) in rats fed low dietary iron (10 mu-g/g), than in rats fed adequate dietary iron (35 mu-g/g). Absorption was greater (p lt 0.05) in SA-, CB- and BT- than Saff-fed rats fed a low iron diet. Enterocyte cytosolic and total cellular iron were less (p lt 0.001) in rats fed low versus adequate dietary iron. In rats fed adequate iron, total cellular iron was dependent (p lt 0.01, r-2=0.59) on liver and serum iron concentrations. Low dietary iron (10 mu-g/g) resulted in iron deficiency, higher absorption, and lower enterocyte c-acon activity and iron concentration than adequate dietary iron (35 mu-g/g). This suggests that enterocyte c-acon activity may be an index of 1RP1 activity and important in enterocyte cellular iron metabolism and absorption.

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Accession: 002801580

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DOI: 10.1016/0271-5317(96)00097-8

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