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Effect of phalloidin on the stability of F- and G-actin



Effect of phalloidin on the stability of F- and G-actin



Molecular Biology New York 29(3 part 2): 349-352



The effect of phalloidin on the stability of monomeric (G) and polymeric (F) actin was assessed using the intrinsic tryptophan fluorescence of the protein and the fluorescence of the hydrophobic probe bis-ANS. The latter was sensitive to actin polymerization. Phalloidin (1-2 molecules/actin molecule) raised the F-actin denaturation temp. by ~15 degrees C. Stabilization of F-actin by phalloidin was more pronounced during denaturation with urea.

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