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Effect of photoperiod on tissue culture of vegetative and flowering-induced plants of Kalanchoe blossfeldiana


, : Effect of photoperiod on tissue culture of vegetative and flowering-induced plants of Kalanchoe blossfeldiana. Revista Brasileira de Botanica 17(1): 19-23

(Effect of photoperiod on tissue culture of vegetative and flowering-induced plants of Kalanchoe blossfeldiana) - Different kinds of explanted tissues from vegetative, flower-induced and flowering plants of Kalanchoe blossfeldiana were cultivated in vitro in dark, short (8h) and long (16h) day conditions. Their ability for shoot regeneration in MS-62 basal medium containing LAA (1.0 mg/l) plus BAP (0.5 mg/1) and callus formation in medium with 2,4-D (0.5 mg/l) plus BAP (0.1 mg/l) were compared. Stem and flower scape segments showed higher morphogenetic potential than leaf discs, independent on the floral state of the donor plant. The degree of bud regeneration increased inversely in relation with the daylength, being higher in the dark, in all kinds of explants. The callus development showed a similar pattern in leaf discs, whereas in stem and flower scape segments the best response was observed when the light period was increased.

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Related references

Lange, O.L.; Schwemmle, B., 1960: Studies on the heat resistance of vegetative and flowering plants of Kalanchoe blossfeldiana. Leaves were severely damaged when heated for 30 minutes at 47 degrees C. on plants in the vegetative phase, and at 50 degrees on those in the flowering phase. After 10 days of short-day treatment, which had a scarcely noticeable effect on leaf suc...

Dickens, C.W.S.; Van-Staden, J., 1988: The in vitro flowering of kalanchoe blossfeldiana poellniz. i. role of culture conditions and nutrients. Nodal explants of Kalanchoe blossfeldiana Poellniz. were cultured in vitro on a low nutrient hormone-free medium. Flowering was achieved in response to short-day inductive cycles. This system was used to test the influence, on the flowering respon...

Ireland, C.R.; Schwabe, W.W., 1982: Studies on the role of photosynthesis in the photoperiodic induction of flowering in the short day plants kalanchoe blossfeldiana and xanthium pensylvanicum 2. the effect of chemical inhibitors of photosynthesis. The role of photosynthesis in flower induction in the short-day plants K. blossfeldiana and X. pennsylvanicum was investigated by chemical suppression of photosynthesis and prevention of chlorophyll formation in the induced leaf. Bleaching leaves...

Sengupta S.K.; Lee C T.; Moore H.T.Jr, 1981: The effect of photoperiod and growth regulator on the development of kalanchoe blossfeldiana in the tropics. Hortscience 16(3 SECT 2): 409

Yang SoonJa; Amaki, W.; Higuchi, H., 2000: The comparative studies on photoperiodic flowering responses of in vitro and in vivo plants in Kalanchoe blossfeldiana Poelln. In in vitro-cultured Kalanchoe blossfeldiana cv. Singapore, the minimum number of short-day cycles required for flowering and the effect of explant size and long-day treatment duration before flower induction on the flowering response were compare...

Alibert G.; Brulfert J.; Boudet A.M., 1981: Effect of photoperiod on enzymes involved in poly phenol biosynthesis in kalanchoe blossfeldiana. The concentration of tannins in young Kalanchoe leaves decreased and malate accumulated when plants were transferred from long to short days. Metabolic fluxes and enzyme capacities involved in the shikimate pathway and in phenolic biosynthesis wer...

Ireland, C.; Schwabe, W., 1982: Studies on the role of photosynthesis in the photoperiodic induction of flowering in the short-day plants Kalanchoe blossfeldiana Poellniz and Xanthium pensylvanicum Wallr. II. The effect of chemical inhibitors of photosynthesis. Journal of experimental botany1892 33(135): 748-760

Janistyn B., 1981: Succulence induction in kalanchoe blossfeldiana under long day conditions by a lipophilic fraction from flowering kalanchoe and mass spectrometric identification of pterosterone. Succulence-induction in K. blossfeldiana is demonstrated under long-day condition by application of a lipophilic fraction from flowering Kalanchoe. Different preparative TLC separation steps yielded a partial purification of this fraction and the...

Younis, A.F., 1955: Studies on the photoperiodism of Kalanchoe blossfeldiana. (II.) A. Flowering of Dutch variety. B. Flowering of cuttings under short-days. Old plants did not flower under long-day conditions or when given 4 short-day cycles of 9 hours light per day but they flowered with abnormal inflorescences when given 5-7 cycles and with normal inflorescences when given 10 or more. Increasing the...

Anonymous, 1955: Studies on the photo periodism of Kalanchoe blossfeldiana II A Flowering of the Dutch variety B Flowering of cuttings under short-day