Effects of EGF on sperm penetration, in vitro maturation, fertilization and development of bovine follicular oocytes
Im, K.S.; Park, K.W.; Kim, H.J.; Chung, K.M.
Sustainable animal production and the environment Proceedings of the 7th AAAP Animal Science Congress, Bali, Indonesia, 11-16 July, 1994 Volume 2 contributed papers: 203-204
1994
Accession: 002820122
Cumulus-oocyte complexes from Holstein cows were cultured for 24 h in TCM 199 medium with HEPES buffer, and were inseminated with spermatozoa suspended in mTALP. Fertilized embryos were cocultured with granulosa cells in TCM 199.
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