+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture

Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture

American Journal of Clinical Nutrition 66(2): 398-405

This study investigated whether naturally occurring garlic derivatives and synthetic S-cysteinyl compounds that resemble garlic constituents have antiproliferative effects on human prostate carcinoma (LNCaP) cells. Studies also examined whether S-allylmercaptocysteine and S-allylcysteine affect two important molecular targets, namely reduced glutathione and polyamines. Results showed that S-allylmercaptocysteine (50 mg/L) diminished LNCaP cell growth whereas the antiproliferative effect of S-allylcysteine was not as pronounced. Studies using synthetic S-cysteinyl analogues revealed that growth inhibition was most effective with compounds containing a disulfide or an active diallyl moiety. Marginal to no inhibitory effect was observed with monosulfinic analogues. Both S-allylmercaptocysteine and S-allylcysteine caused an increase in LNCaP cell reduced glutathione concentrations. Putrescine and spermine concentrations decreased and spermidine increased 3 d after S-allylmercaptocysteine treatment. At 5 d after S-allylmercaptocysteine treatment, polyamine concentrations were similar to those of saline-treated controls. Diminished cell growth and altered polyamine concentrations suggest that S-allylmercaptocysteine may impede the polyamine synthesizing enzyme, ornithine decarboxylase, either by enhancing the formation of reduced glutathione, a known inhibitor of ornithine decarboxylase, or by reacting directly with ornithine decarboxylase at its nucleophilic thiol moiety. Because S-allylcysteine also increases reduced glutathione formation but does not significantly inhibit growth, the latter mechanism may be more likely for this compound. These data provide further evidence that nonessential nutrients derived from garlic may modulate tumor growth. Further research is required on effects of garlic derivatives in vivo before information from the present studies can be used to assist in the development of effective nutritional strategies for preventing progression of prostate cancer.

(PDF emailed within 0-6 h: $19.90)

Accession: 002822275

Download citation: RISBibTeXText

PMID: 9250120

DOI: 10.12968/bjon.1997.6.11.654

Related references

Tissue culture of the human prostate--effects of sex hormones on subcultures of cells from benign prostatic hyperplasia and carcinoma of the prostate. Nihon Hinyokika Gakkai Zasshi. Japanese Journal of Urology 69(3): 261-284, 1978

Antiproliferative effects of the MGBG derivative polyamine biosynthesis inhibitor CGP 48664A against human melanoma, colon, prostate and breast carcinoma cells. Clinical & Experimental Metastasis 12(5): 94, 1994

Effects of L-valine on growth and polyamine metabolism in human colon carcinoma cells. Biochimica Et Biophysica Acta. 1379(1): 151-160,. 8, 1998

Effects of cyclosporine in combination with alpha-difluoromethylornithine on growth and polyamine metabolism of human T-leukaemia cells in culture. Biochemical Society Transactions 18(6): 1226-1226, 1990

In vivo and in vitro effects of selenium-enriched garlic on growth of human gastric carcinoma cells. Zhonghua Zhong Liu Za Zhi 23(6): 461-464, 2002

Antitumor activity of the polyamine analog N1,N11-diethylnorspermine against human prostate carcinoma cells. Prostate 44(4): 313-321, September 1, 2000

Sensitivity to polyamine-induced growth arrest correlates with antizyme induction in prostate carcinoma cells. Cancer Research 59(24): 6109-6112, 2000

Effects of agmatine on polyamine metabolism and the growth of prostate tumor cells. Journal of Biochemistry & Molecular Biology 32(2): 173-180, March 31, 1999

Effects of calcium ion concentration on growth of human epidermal cells in culture. Journal of Investigative Dermatology 74(4): 257, 1980

Biochemical effects and growth inhibition in MCF-7 cells caused by novel sulphonamido oxa-polyamine derivatives. CMLS Cellular & Molecular Life Sciences 59(4): 715-723, April, 2002

Inhibitory effects of selenium on the growth of DU-145 human prostate carcinoma cells in vitro. Biochemical and Biophysical Research Communications 130(2): 603-609, 1985