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Effects of growth regulators, sucrose, light, and medium on callus and shoot formation in shoot-tip culture of Hibiscus syriacus L. 'Honghwarang'



Effects of growth regulators, sucrose, light, and medium on callus and shoot formation in shoot-tip culture of Hibiscus syriacus L. 'Honghwarang'



Journal of the Korean Society for Horticultural Science 37(2): 317-323



The effects of plant growth regulators (NAA, 2,4-D, kinetin, BA or thidiazuron), sucrose (2, 4, 6 or 8%), light (0, 16 or 24 h light/24 h) and media (MS, NH, N6 or B5) on the production of callus and shoots of H. syriacus and on plant regeneration were investigated. Callus induction and growth was promoted in the presence of 2% sucrose and 16 h light. The best medium for callus induction was half-strength MS.

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