Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance
Bengtsson, U.; Knutson, T.W.; Knutson, L.; Dannaeus, A.; Hällgren, R.; Ahlstedt, S.
Journal of Allergy and Clinical Immunology 100(2): 216-221
ISSN/ISBN: 0091-6749 PMID: 9275143 DOI: 10.1016/s0091-6749(97)70227-6
Five patients (mean age 54 years) with a history of milk-related diarrhoea but with a negative response to skin prick tests and radioallergosorbent assays with milk and 8 healthy control subjects (mean age 28 years) were studied to determine the local intestinal secretion of eosinophil cationic protein (ECP) and histamine. Perfusion studies were carried out using cow milk, casein and whey as antigens. Luminal milk induced an increase in secretion of histamine and ECP in the study group during the first 20 min after challenge. Histamine concentration increased from 123+or-12 to 543+or-175 ng/cm per h and ECP from 80+or-23 to 686+or-262 ng/cm per h. Similar results were not obtained with casein or whey or in the control subjects.