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Establishment of embryogenic cell suspension cultures and development of somatic embryo on Camellia sinensis var. assamica Kitamura. I. Establishment of embryogenic suspension cell line


Establishment of embryogenic cell suspension cultures and development of somatic embryo on Camellia sinensis var. assamica Kitamura. I. Establishment of embryogenic suspension cell line



Journal of Beijing Forestry University, English edition 5(1): 65-74



Calluses derived from mature cotyledons of C. sinensis var. assamica were subcultured in modified MS agar medium supplemented with 0.05 mg 2,4-D and 0.5 mg 6-BA litre. Various kinds of calluses were formed after many subcultures, including yellow friable callus (YF), yellow semi-transparent callus (YS), green semi-transparent callus (GS), green hard callus (GH) and white hard callus (WH).

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