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Evaluation of immunoprecipitation techniques in chromoblastomycosis



Evaluation of immunoprecipitation techniques in chromoblastomycosis



Journal de Mycologie Medicale 6(2): 83-87



The present studies aimed at the development and validation of an antigen-reagent for the routine serodiagnosis of chromoblastomycosis caused by Cladosporium carrionii. This chronic and often crippling disease has a high incidence in some areas of Venezuela, but, to our knowledge, no commercial reagents are currently available for its serodiagnosis. The strain UNEFM-PP-8201 of C. carrionii, originally isolated from a patient from the endemic area of the Falcon State (Venezuela), was used for antigen extraction. The fungus was grown in neutral-glucose-peptone (NGP, either broth or agar), and metabolic (AgMPP) and somatic (AgSPP) antigens were harvested at the exponential growth phase. Rabbit hyperimmune sera were obtained against these antigenic fractions to investigate their antigenic composition and to standardize the immunoprecipitation techniques. A total of 71 human sera were included in this work, of which, 20 belonged to patients with chromoblastomycosis caused by C. carrionii, 12 to patients with chromoblastomycosis caused by Fonsecaea pedrosoi, 29 to patients with other mycosis and 10 to healthy subjects. All sera were tested by double diffusion (DD) and counter immunoelectrophoresis (CIE). In DD, AgMPP showed high specificity (95%) and low sensitivity (50%), whereas AgSPP was very highly specific (100%) and more sensitive (70%). In CIE, AgMPP demonstrated high sensitivity (95%) but poor specificity (53%), while AGSPP showed low sensitivity (10%) and high specificity (97%). Thus, DD, using AgSPP, appears to be the method of choice for the serologic diagnosis of chromoblastomycosis caused by C. carrionii.

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