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Expression of a reporter gene in insect cells by a new transfer vector with baculovirus early promoter



Expression of a reporter gene in insect cells by a new transfer vector with baculovirus early promoter



Chinese Journal of Virology 12(2): 170-176



Using the IE1 gene promoter of Autographa californica nuclear polyhedrosis virus (AcNPV), a transfer vector, pAcPIneo, was constructed. This contains a neomycin resistance gene (neo) coding sequence downstream of an immediately early gene (IE1) promoter. It was cotransfected with wild type AcNPV DNA into Spodoptera frugiperda Sf9 insect cells.

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