Amylose content of milled rice is a significant quality parameter. Genetic analysis of amylose content was conducted using single-grain amylose analysis in F2 hybrids from the crosses Lemont x Taichung Sen 10, Rexmont x Taichung Sen 10 and Guichow x Lemont. Intermediate to low amylose content appeared to be controlled by a single incompletely dominant gene in the combinations between the intermediate (Lemont) and low (Taichung Sen 10) amylose parents. Two incompletely dominant complementary genes conditioning high over low were suggested from the combinations between high (Rexmont, Guichow) and low amylose parents. A genetic hypothesis of a single incompletely dominant gene controlling high to intermediate amylose content was fitted in mating high with intermediate amylose parents. Evidence that amylose content was also influenced by genes with minor effects or modifiers was observed in some combinations.