EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of Mycobacterium paratuberculosis gene expression signals


, : Identification of Mycobacterium paratuberculosis gene expression signals. Microbiology 143: 921-928

Mycobacterium paratuberculosis promoter-containing clones were isolated from a genomic DNA library constructed in the transcriptional-translational fusion vector pYUB76. The promoter-containing DNA fragments were identified in the surrogate host Mycobacterium smegmatis by expression of the promoterless lacZ reporter gene of pYUB76. The expression signals exhibited a wide range of strengths, as indicated by their corresponding beta-galactosidase activities. Eight clones were sequenced and characterized further. Predicted open reading frames and codon usage were identified by computer analysis. Database searching for related sequences using the BLAST method revealed no homologies. Transcriptional activity was measured by slot-blot hybridization with steady-state RNA isolated from lacZ+ M. smegmatis clones. Primer extension analysis identified the transcription start sites within the cloned fragments. The promoter regions characterized in this study were used to establish a consensus promoter sequence for M. paratuberculosis. M. paratuberculosis consensus hexanucleotide sequences of TGMCGT and CGGCCS centred approximately 35 and 10 bp upstream from the transcription startpoints do not correspond to the consensus hexanucleotides of Escherichia coli promoters.

(PDF same-day service: $9.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863021

PMID: 9084176

DOI: 10.1099/00221287-143-3-921

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Malorni, W.; Rainaldi, G.; Rivabene, R.; Santini, M.T.; Peterson, S.W.; Testa, U.; Donelli, G., 1994: Cytoskeletal oxidative changes lead to alterations of specific cell surface receptors. Electron acceptors in biological systems, termed "oxidants", have been associated with numerous cytopathic conditions. In particular, oxygen-derived free radicals have been shown to induce various subcellular oxidative injuries which can...

Anonymous, 2013: Reduction of help before dispersal. In cooperative breeders, sexually mature subordinates can either queue for chances to inherit the breeding position in their natal group, or disperse to reproduce independently. The choice of one or the other option may be flexible, as when indivi...

Ilnicki, P., 1984: Effect of peatland use on the physical properties of the topsoil. Data are presented on the effects of drainage on bulk density, porosity and soil water retention.

Bloom, S.R.; Barnes, A.J.; Bryant, M.G.; Alberti, K.G., 1976: Plasma glucagon in diabetes resulting from total pancreatectomy [proceedings. Metabolism: Clinical and Experimental 25(11 Suppl 1): 1481-1482

Deng, H.; Hughes, S.C.; Bell, J.B.; Simmonds, A.J., 2008: Alternative requirements for Vestigial, Scalloped, and Dmef2 during muscle differentiation in Drosophila melanogaster. Vertebrate development requires the activity of the myocyte enhancer factor 2 (mef2) gene family for muscle cell specification and subsequent differentiation. Additionally, several muscle-specific functions of MEF2 family proteins require binding...

Rose M.E.; Lawn A.M.; Millard B.J., 1984: The effect of immunity of the early events in the life cycle of eimeria tenella in the cecal mucosa of the chicken. A comparison was made between the early events (asexual stages) in the life-cycle of E. tenella in specifically immunized and control chickens. Particular attention was paid to the quantitative aspects and to the transport of sporozoites within in...

Kirkman H.N.; Galiano S.; Gaetani G.F., 1987: The function of catalase bound nadph. Catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6) is of historical interest for having been the subject of some of the earliest investigations of enzymes. A feature of catalase that has been poorly understood for several decades, however, is the me...

Ingram Roy Lee, 1953: Clay minerals of the Tuscaloosa Formation near Fayetteville, North Carolina. The Journal of the Elisha Mitchell Scientific Society 69(2): 92

Piotti, P.; Genitoni, V.; Comazzi, R.; Pagnoni, A.M.; Guindani, A.; Cerasoli, S.; Villani, F., 1984: Relationship between pulmonary function tests and morphologic changes in the lung in bleomycin-treated patients. The transfer factor of the lung for carbon monoxide (TLCO) and the respiratory response to moderate exercise were determined in 16 patients with pulmonary metastasis pretreated with bleomycin who underwent pulmonary partial resection. The results...

Peacock, M.J.; Wolstencroft, J.H., 1976: Projections of reticular neurones to dorsal regions of the spinal cord in the cat. Reticulospinal neurones in the cat were identified by extracellular recording and antidromic stimulation of their axons in the cord. Approximately 34% of reticulospinal neurones in the medulla, and 28% in the pons, were found to project to dorsal...