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Identification of Pseudomonas aeruginosa on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms


, : Identification of Pseudomonas aeruginosa on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Acta Microbiologica Polonica 44(2): 111-117

Forty 40 clinical strs of Pseudomonas aeruginosa were investigated by using the polymerase chain reaction (PCR). Primers were used to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci. When the spacer amplification products were resolved by electrophoresis, the resulting patterns were characteristic for all tested strs. Only one specific PCR fragment of 580 bp was formed. This product was digested with HaeIII, HinII and AluI restriction endonucleases.

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Accession: 002863039

PMID: 8906931

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