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Identification of Pseudomonas aeruginosa on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms


Acta Microbiologica Polonica 44(2): 111-117
Identification of Pseudomonas aeruginosa on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms
In the present study, 40 clinical strains or Pseudomonas aeruginosa were investigated by using the polymerase chain reaction (PCR). We used primers to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci. When the spacer amplification products were resolved by electrophoresis, the resulting patterns were characteristic for all tested strains. Only one specific PCR fragment or 580 bp was formed. This product was digested with HaeIII, HinfI and AluI restriction endonucleases. Restriction fragments produced by all three restriction endonucleases were characteristic and have the same sizes for all strains tested. The amplification product contains a conserved, internal single HadII restriction site. On the basis of our results, PCR amplifications of the 16S-23S spacer region for Pseudomonas aeruginosa and subsequent RFPL analysis show significant promise as a tool for the simple identification of this bacteria.


Accession: 002863039

PMID: 8906931



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