+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of RAPD markers for 11 Hessian fly resistance genes in wheat

, : Identification of RAPD markers for 11 Hessian fly resistance genes in wheat. Theoretical & Applied Genetics 94(3-4): 419-423

The pyramiding of genes that confer race- or biotype-specific resistance has become increasingly attractive as a breeding strategy now that DNA-based marker-assisted selection is feasible. Our objective here was to identify DNA markers closely linked to genes in wheat (Triticum aestivum L.) that condition resistance to Hessian fly (Mayetiola destructor (Say)). We used a set of near-isogenic wheat lines, each carrying a resistance gene at 1 of 11 loci (H3, H5, H6, H9, H10, H11, H12, H13, H14, H16 or H17) and developed by backcrossing to the Hessian fly-susceptible wheat cultivar 'Newton'. Using genomic DNA of these 11 lines and 'Newton', we have identified 18 randomly amplified polymorphic DNA (RAPD) markers linked to the 11 resistance genes. Seven of these markers were identified by denaturing gradient gel electrophoresis and the others by agarose gel electrophoresis. We confirmed linkage to the Hessian fly resistance loci by cosegregation analysis in F-2 populations of 50-120 plants for each different gene. Several of the DNA markers were used to determine the presence/absence of specific Hessian fly resistance genes in resistant wheat lines that have 1 or possibly multiple genes for resistance. The use of RAPD markers presents a valuable strategy for selection of single and combined Hessian fly resistance genes in wheat improvement.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863046

DOI: 10.1007/s001220050431

Download PDF Full Text: Identification of RAPD markers for 11 Hessian fly resistance genes in wheat

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Dowling, R.J.; Laing, A.D.; Vincent, J.M., 1999: Imaging and stenting for renal artery stenosis. Renal artery stenosis is a potentially correctable cause of hypertension and renal failure, using endoluminal or, less commonly, surgical techniques. A number of imaging techniques can be used to diagnose renal artery stenosis, all with similar ac...

Pencharz, P.B., 1988: The 1987 Borden Award lecture. Protein metabolism in premature human infants. Our studies have focused on the regulation of whole body and skeletal muscle protein metabolism in premature infants. Net deposition of protein is the result of a positive balance between protein synthesis and breakdown. To measure protein metabol...

Northup, P.G.; Argo, C.K.; Al-Osaimi, A.M.; Schmitt, T.M., 2007: Serum sodium levels are not independent of fluid status and disease severity in liver transplant waiting list candidates. Hepatology 46(4): 485A

Kehar, N.D.; Mccollum, E.V., 1934: Bound water in cardiac muscle in relation to ventricular fibrillation. Samples of ventricular muscle from the perfused dog heart were taken before and 5 min. after establishment of ventricular fibrillation by electrical stimulation, and analyzed by Sayre's calorimetric method. The av. results of 6 exps. showed a...

Berntsen, K.Norgård., 2011: Trends in total and cause-specific mortality by marital status among elderly Norwegian men and women. Previous research has shown large and increasing relative differences in mortality by marital status in several countries, but few studies have considered trends in cause-specific mortality by marital status among elderly people. The author uses d...

Pavel Y.G.; Fedotovskii A.N.; Val'dman E.K.; Pavel E.A.; Meel A.Yu, 1985: Determination of the specific weight of the factorial characters of general resistance. The genetic viability of roosters was determined on the basis of the study of the immunological characters of their female chicks. Apparently, universal principal general resistance characters do not exist. The specific weight of factorial charact...

Brody, G.S., 1977: Fact and fiction about breast implant "bleed". Plastic and Reconstructive Surgery 60(4): 615-616

Carpenter, D-Ann.L.; Kamaka, M.L.; Kaulukukui, C.Malina., 2013: An innovative approach to developing a cultural competency curriculum; efforts at the John A. Burns School of Medicine, Department of Native Hawaiian Health. Initial efforts to teach cultural competency at the University of Hawai'i John A. Burns School of Medicine began in the late 1990s through the Native Hawaiian Center of Excellence. With the formation of the Department of Native Hawaiian Healt...

Allen,T.T.; Han,H.S.; Shook,S.R., 2008: A structural assessment of the contract logging sector in the Inland Northwest. Logging contractors supply raw material (i.e., sawlogs and wood chips) to the forest products manufacturing sector and are an integral component of the Inland Northwest's forest products industry. However, a lack of information exists describ...

Zeuzem, S.; Piiper, A.; Stryjek Kaminska, D.; Stein, J.; Caspary, W.F., 1993: The effect of synthetic peptides of the rab3A effector domain on basal and cholecystokinin-octapeptide stimulated amylase release from digitonin-permeabilized pancreatic acini. Gastroenterology 104(4 SUPPL ): A346