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Identification of RAPD markers linked to linolenic acid genes in rapeseed






Euphytica 90(3): 351-357

Identification of RAPD markers linked to linolenic acid genes in rapeseed

The inheritance of the low linolenic acid content (derived from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high times low linolenic acid F-1 hybrid. 'Bulked segregant analysis' made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independent mutations were implied in the low linolenic acid content.

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Accession: 002863052



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