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Identification of RAPD markers linked to linolenic acid genes in rapeseed



Identification of RAPD markers linked to linolenic acid genes in rapeseed



Euphytica 90(3): 351-357



The inheritance of the low linolenic acid content (derived from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high times low linolenic acid F-1 hybrid. 'Bulked segregant analysis' made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independent mutations were implied in the low linolenic acid content.

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Accession: 002863052

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