EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR






Journal of Clinical Microbiology 35(2): 462-467

Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR

A PCR system for the detection and identification of group IV spirochetes (Serpulina pilosicoli) was designed to complement biochemical tests, e.g., the hippurate hydrolysis and beta-glucosidase tests, and to verify the accuracy of a previously proposed biochemical classification system. The PCR assay was based on amplification of a segment of the 16S rRNA gene. Both primers were constructed to selectively amplify the 16S rRNA gene of Serpulina pilosicoli. All analyzed Serpulina strains exhibiting the capacity to hydrolyze hippurate and lacking beta-glucosidase activity, including the type strain for spirochetal diarrhea, P43, were amplified with the PCR system. All other tested strains, including type and field strains of different phenotypes of Serpulina species, as well as Salmonella species, Campylobacter species, and Escherichia coli strains, were negative in the assay. Among the tested strains were 18 Scottish field isolates originating from the mucosae of pigs with colitis. A simple classification scheme, suitable for routine classification of porcine intestinal spirochetes, is also presented. The scheme is based on hemolysis, indole production, and the hippurate hydrolysis test.

(PDF 0-2 workdays service: $29.90)

Accession: 002863065

PMID: 9003616



Related references

Rapid identification of porcine Serpulina species. Veterinary Record 140(2): 51-52, 1997

Rapid identification of porcine Serpulina species by colony blot assay using a genus-specific monoclonal antibody. Veterinary Record 139(22): 539-541, 1996

The phylogeny of intestinal porcine spirochetes (Serpulina species) based on sequence analysis of the 16S rRNA gene. Journal of Bacteriology 178(14): 4189-4199, 1996

Investigations into field cases of porcine colitis with particular reference to infection with Serpulina pilosicoli. Veterinary Record 142(10): 235-239, 1998

Biochemical and serological properties of porcine Serpulina isolates. Medycyna Weterynaryjna 53(1): 33-37, 1997

Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. International Journal of Systematic Bacteriology 42(1): 189-190, 1992

Identification of porcine Serpulina strains in routine diagnostic bacteriology. Magyar Allatorvosok Lapja 121(6): 337-339, June, 1999

Identification of porcine Serpulina strains in routine diagnostic bacteriology. Veterinary Microbiology 62(2): 163-169, 1998

Identification of weakly beta-hemolytic porcine spirochetes by biochemical reactions, PCR-based restriction fragment length polymorphism analysis and species-specific PCR. Journal of Veterinary Medical Science 70(8): 837-840, 2008

Laboratory identification and enteropathogenicity testing of Serpulina pilosicoli associated with porcine colonic spirochetosis. Journal of Veterinary Diagnostic Investigation 9(2): 165-171, 1997