EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR


, : Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. Journal of Clinical Microbiology 35(2): 462-467

A PCR system for the detection and identification of group IV spirochetes (Serpulina pilosicoli) was designed to complement biochemical tests, e.g., the hippurate hydrolysis and beta-glucosidase tests, and to verify the accuracy of a previously proposed biochemical classification system. The PCR assay was based on amplification of a segment of the 16S rRNA gene. Both primers were constructed to selectively amplify the 16S rRNA gene of Serpulina pilosicoli. All analyzed Serpulina strains exhibiting the capacity to hydrolyze hippurate and lacking beta-glucosidase activity, including the type strain for spirochetal diarrhea, P43, were amplified with the PCR system. All other tested strains, including type and field strains of different phenotypes of Serpulina species, as well as Salmonella species, Campylobacter species, and Escherichia coli strains, were negative in the assay. Among the tested strains were 18 Scottish field isolates originating from the mucosae of pigs with colitis. A simple classification scheme, suitable for routine classification of porcine intestinal spirochetes, is also presented. The scheme is based on hemolysis, indole production, and the hippurate hydrolysis test.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002863065

PMID: 9003616

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Pokrovskii, A. A.; Korobkina, G. S.; Danilova, E. N.; Laricheva, K. A.; Levyant, P. P.; Slavgorodskaya, I. P., 1975: Edible protein product. The product contains (% by wt.) sodium citrate 0.9-1.0, potassium citrate 0.9-1.0, magnesium citrate 0.3-0.4, NaHCO3 0.7-0.8, dried skim-milk 20.0-22.0, whole cows' milk 13.0-14.5, cream 3.6-4.0, vegetable oil 1.9-2.1, sucrose 14.0-15.4, edib...

Menachem, M.; Grupper, M.; Paz, A.; Potasman, I., 2008: Assessment of rabies exposure risk among Israeli travelers. Background: The decision whether or not to administer rabies pre-exposure prophylaxis (PEP) to travelers visiting endemic areas is a complex one. Paramount for making that decision is knowledge of the risk of animal bites during travel. This study...

Fishbein, Mark, 2008: A new, diminutive, Mexican milkweed (Asclepias, Apocynaceae s.l.). A new milkweed from the eastern and western cordilleras of Mexico is described and illustrated. Asclepias elegantula Fishbein is distinguished from other diminutive, linear-leaved species of the genus in montane Mexico by the claret-striped petals...

Mcpherson, James C.Jr; Chuang, A. H.; Mcpherson, James C.IIi, 2001: A surfactant that affects blood flow in the microcirculation. Survival of elevated skin flaps depends largely upon maintaining blood flow in the microcirculation of the flap. We have reported a poloxamer surfactant enhancement of early wound healing of incisional wounds. One mechanism of action of the poloxa...

Kim YongWook; Cho JoonHyeong, 2005: Growth and yields of Korean soybean cultivars in drained-paddy field. The growth and yield of several local soyabean cultivars were evaluated in a field experiment conducted in Korea Republic to identify cultivars that are suitable for cultivation in the paddy field. Retarded growth of top plants and roots, and rela...

Clausen C. D.; Leuteritz K.; Ziegler W., 1987: The eastern provincial quarry at Drewer (northeastern Rhenish Slate Mountains), a stratotype candidate for the Devonian/ Carboniferous boundary. Senckenbergiana Lethaea 67(5-6): 467-477

Ellory, J.C.; Robinson, H.C.; Browning, J.A.; Stewart, G.W.; Gehl, K.A.; Gibson, J.S., 2007: Abnormal permeability pathways in human red blood cells. A number of situations that result in abnormal permeability pathways in human red blood cells (RBCs) have been investigated. In sickle cell disease (SCD), RBCs contain HbS, rather than the normal HbA. When deoxygenated, an abnormal conductance pat...

Mujeriego, R.; Compte, J.; Cazurra, T.; Gullón, M., 2008: The water reclamation and reuse project of El Prat de Llobregat, Barcelona, Spain. Water reclamation and reuse have become essential components of water resources management in the Metropolitan Area of Barcelona, by helping to develop additional water resources in the lower Llobregat River, one of its main sources of water supp...

Rattarasarn, C.; Diosdado, M.; Sunthornpun, S., 1997: Latent autoimmune diabetes in Thai NIDDM patients with secondary oral hypoglycemia agents failure. Diabetologia 40(SUPPL 1): A74

Jung, 1945: Ernahrungsphysiologie der Hauptnahr-stoffgruppen. Mitteil Geb Lebensmitteluntersuch U Hyg Eidgen Gesundheits 36(2/3): 109-136