+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting

, : Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting. Journal of Dairy Science 80(3): 471-476

Identification of Streptococcus species isolated from bovine milk by randomly amplified polymorphic DNA fingerprinting was evaluated. Bacterial DNA templates were prepared using a standardized method for randomly amplified polymorphic DNA amplification and previously evaluated arbitrary primers. Amplified DNA fragments were visualized by agarose gel electrophoresis and were analyzed by densitometry. Unidentified streptococci (n = 163) that were isolated from mammary secretions of dairy cows were evaluated. The DNA fingerprint patterns of unidentified bacteria were compared using a computerized database that contained DNA fingerprint patterns of test strains. Comparison with the API 20 Strep identification system (bioMérieux Vitek, Inc., Hazelwood, MO) and conventional biochemical tests showed that about 91% of isolates (148 of 163) were identified correctly by DNA fingerprinting. The sensitivity of the DNA fingerprinting technique was 90%, and the specificity was 92%. However, the DNA fingerprinting technique only identified 4 of the 11 species included in the study. Three of the 4 species, Streptococcus uberis, Streptococcus agalactiae, and Streptococcus dysgalactiae, represent the streptococci isolated most frequently from cows with mastitis. The other Streptococcus and Enterococcus species that were not identified by the DNA fingerprinting system are less frequently isolated as causative agents of mastitis. Expanding the DNA fingerprint database would likely increase the sensitivity and specificity of this technique.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002863068

PMID: 9098796

DOI: 10.3168/jds.S0022-0302(97)75959-9

PDF Full Text: Identification of Streptococcus species by randomly amplified polymorphic deoxyribonucleic acid fingerprinting

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Trenckmann, H.; Kubel, M.; Rosendranz, M., 1976: Problems of idiopathic congestive cardiomyopathy. It is reported on the course of an idiopathic congestive cardiomyopathy in a younger man which could be observed during three years. After a longer period free of symptoms acutely appeared the signs of a myocardial failure, in which case disturban...

Yates, W.D.; Chatman, D.M.; Rogers, N.; Chung, E.B.; Nunez, A.; Leffall, L.D., 1992: Hyperparathyroidism in black patients: an update. Cervical exploration with removal of the pathologic gland or glands is effective treatment for parathyroid adenomas and hyperplasia. This article reports the results of a retrospective study of 41 patients who underwent elective cervical explorati...

Elthon D., 1978: The generation and evolution of marginal basin basalts; the perspective from an ophiolite formed in a marginal basin. Eos, Transactions, American Geophysical Union 59(12): 1191

Houle, S.; Dasilva, J.N.; Wilson, A.A., 2000: Imaging the 5-HT1A receptors with PET: WAY-100635 and analogues. This paper summarizes our work on WAY-100635 and analogues, labeled either with carbon-11 or fluorine-18, as potential radioligands for the 5-hydroxytryptamine1A (5-HT1A) neuroreceptors. Other facets of our work include: (1) human studies with (O-...

Pastor M.; Zienkiewicz O.C.; Chan A.H.C., 1990: Generalized plasticity and the modelling of soil behaviour. Yield and plastic potential surfaces need not be explicitly defined in the theory of generalized plasticity outlined in this paper and a very effective general model describing the behavior of sands and of clays under monotonic or transient is loa...

Kreimeier U.; Sonnenberg K.; Schwarz R., 1990: Limulus amoebocyte lysate lal assay standardization of test procedure with chromogenic substrate and validation in experimental endotoxemia. Circulatory Shock 31(2): 251-252

Uhlig H., 1990: Processing of fruit vegetables and wine. Gerhartz, W (Ed ) Enzymes in Industry: Production And Applications Xvii+321p Vch Verlagsgesellschaft Mbh: Weinheim, Germany; Vch Publishers, Inc : New York, New York, Usa Illus 126-128

Hu, L.M.; Davies, P.; Adzick, N.S.; Harrison, M.R.; Reid, L.M., 1987: The effects of intrauterine pneumonectomy in lambs. A morphometric study of the remaining lung at term. A morphometric study was made of the remaining lung of term lambs that had undergone left pneumonectomy at 60, 80, 100, or 120 days gestation. All pneumonectomy lambs showed some compensatory growth since right lung volume to body weight ratio was...

Cheng, J.W.M., 2006: Use of non-aspirin nonsteroidal antiinflammatory drugs and the risk of cardiovascular events. OBJECTIVE: To review published evidence on the use of nonselective, non-aspirin nonsteroidal antiinflammatory drugs (NANSAIDs) and the risks of cardiovascular events and examine the justification for Food and Drug Administration (FDA) in recommen...

Panossian, A.; Hovhannisyan, A.; Mamikonian, G.; Abrahamian, H.; Goukasova, G.; Gabrielian, E.; Wikman, G.; Wagner, H., 2000: Pharmacokinetic andrographolide from Andrographis paniculata extract SHA-10 and Andrographis fixed combination Kan Jang in rats and humans. Phytomedicine (Jena) 7(Supplement 2): 118