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Identification of WYMV, WSSMV and BaYMV using monoclonal antibodies against the French isolate of WYMV


, : Identification of WYMV, WSSMV and BaYMV using monoclonal antibodies against the French isolate of WYMV. Agronomie 15(7/8): 511

Analysis of wheat yellow mosaic bymovirus, wheat spindle streak mosaic bymovirus and barley yellow mosaic bymovirus isolates by IDAS and ACP ELISA showed that none of the monoclonal antibodies of the French WYMV isolate had a complete specificity to these bymoviruses.

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Related references

Hariri, D.; Delaunay, T.; Gomez, L.; Filleur, S.; Plovie, C.; Lapierre, H., 1995: Identification of the WYMV, the WSSMV, and the BaYMV through monoclonal antibodies directed against the French isolate of the WYMV. Agronomie (Paris) 15(7-8): 511

Hariri, D.; Delaunay, T.; Gomes, L.; Filleur, S.; Plovie, C.; Lapierre, H., 1996: Comparison and differentiation of wheat yellow mosaic virus (WYMV), wheat spindle streak mosaic virus (WSSMV) and barley yellow mosaic virus (BaYMV) isolates using WYMV monoclonal antibodies. Twelve monoclonal antibodies (MAbs) were obtained by immunizing mice with a French isolate (F1) of wheat yellow mosaic bymovirus (WYMV). Three of these (3D12, 2C1, 6C3) belong to the IgM class and the 9 others to the IgG class (3D8, 3H1, 2B8, 1F2,...

Lapierre, H.; Prud' homme, H.; Fouchard, H.; Lebrun, L.; Hariri, D., 1995: Assistance to long-distance transport between wheat yellow mosaic virus (WYMV) and soil-borne wheat mosaic virus (SBWMV) in bread wheat cultivars resistant to WYMV. Interactions between wheat yellow mosaic bymovirus and wheat soil-borne mosaic furovirus in wheat and behaviour of various wheat cultivars to these viruses in soil under lab. and field conditions were studied in France.

Cheng Ye; Chen Jiong; Yang JianPing; Chen JianPing, 1999: Cloning and sequence NIb gene of Chinese soil-borne WYMV Pujiang isolate. A 1.6 kb fragment, which contained the complete sequence of the NIb gene of wheat yellow mosaic virus (WYMV) (collected from an infected field in Pujiang, Zhejiang Province, China) was amplified by RT-PCR with a pair of primers (NIb01 and NIb02) w...

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Lin, M.C.; Ruan, Y.L., 1986: On the wheat yellow mosaic virus (WYMV). In wheat with yellow mosaic symptoms 1 to several inclusion bodies are enveloped in infected surface cells. Virus particles are slightly flexuous filaments, 13 x 100-600 nm, often with pinwheels and aggregates in the cytoplasm. Hosts include 13 Tr...

Chen J P.; Ruan Y L., 1990: Purification serology and other properties of wheat yellow mosaic virus wymv. Wheat yellow mosaic virus (WYMV) has been occurred on wheat in Shanxi province since 1970s. The virus preparation was purified from naturally infected wheat plants by extracting in 0.5 mol/L potassium phosphate buffer pH 7.0, clarifying in 1/4 v c...

YueHongNi; WuYunFeng; LiYiRan; WeiTing; HouWei; WuKeKe, 2008: Simultaneous detection of three wheat virus BSMV, BYDV-PAV, WYMV and WBD phytoplasma by multiplex PCR. Objective: Reaction components and reaction cycling parameters were optimized for establishing a multiplex PCR (M-PCR) system to simultaneous detect <i>barley stripe mosaic virus</i>, <i>barley yellow dwarf virus</i>-PAV, &...

Fouchard, M.; Hariri, D.; Prud' homme, H.; Lebrun, I.; Koenig, J.; Lapierre, H., 1995: Resistance of old French cultivars of bread wheat to soil-borne wheat mosaic virus (SBWMV) and wheat yellow mosaic virus (WYMV). A total of 158 sources of bread wheat, comprising landraces and the first wheat cultivars registered in the French catalogue, was analysed for resistance to wheat soil-borne mosaic furovirus (WSBMV) and wheat yellow mosaic bymovirus (WYMV). Like t...

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