EurekaMag.com logo
+ Translate

Identification of a Plasmodium berghei antigen sharing common features with P. falciparum and P. chabaudi parasitophorous-vacuole membrane antigens


, : Identification of a Plasmodium berghei antigen sharing common features with P. falciparum and P. chabaudi parasitophorous-vacuole membrane antigens. Parasitology Research 82(2): 130-135

On the basis of immunological cross-reactivity, we identified a 43-kDa Plasmodium berghei antigen with homology to the exp-1 antigen from P. falciparium. The P. berghei antigen was recognized by an antibody directed against an epitope on the C-terminus of the P. falciparum exp-1 protein. This antigen is localized on the surface of the parasite and shares peptide sequence homology with the P. chabudi antigen Ag3008. To investigate further the role of the P. berghei antigen, we designed antisense phosphorothioate oligodeoxynucleotides (PS oligos) complementary to sequences of the exp-1 mRNA from P. falciparum. The PS oligos were capable of inhibiting the development of P. falciparum in vitro by 47%. In vivo, experiments in mice showed that the same PS oligos had the potential to extend the life span of mice infected with P. berghei by a factor of 2-4. The immunological cross-reactivity and the antisense inhibition of P. berghei parasite development in vivo indicate that this antigen may be a homologue of exp-1 from P. falciparum that has functional importance for parasite survival.

Accession: 002863103

PMID: 8825207

DOI: 10.1007/s004360050083

Download PDF Full Text: Identification of a Plasmodium berghei antigen sharing common features with P. falciparum and P. chabaudi parasitophorous-vacuole membrane antigens


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Tan, T.M.; Goh, K.L.; Binh, L.N.; Ting, R.C.; Kara, U.A., 1996: Identification of a Plasmodium berghei antigen sharing common features with P. falciparum and P. chabaudi parasitophorous-vacuole membrane antigens. On the basis of immunological cross-reactivity, we identified a 43-kDa Plasmodium berghei antigen with homology to the exp-1 antigen from P. falciparium. The P. berghei antigen was recognized by an antibody directed against an epitope on the C-ter...

Favaloro, J.M.; Culvenor, J.G.; Anders, R.F.; Kemp, D.J., 1993: A Plasmodium chabaudi antigen located in the parasitophorous vacuole membrane. In order to study antigens from the rodent malaria Plasmodium chabaudi, clones reacting with mouse hyperimmune serum were selected from an expression library of blood-stage P. chabaudi cDNAs in the vector pGEX-2T. Sixty-four such clones were shown...

Kara, U.; Murray, B.; Pam, C.; Lahnstein, J.; Gould, H.; Kidson, C.; Saul, A., 1990: Chemical characterization of the parasitophorous vacuole membrane antigen QF 116 from Plasmodium falciparum. On the basis of amino acid sequencing and immunological cross-reactivity, the Plasmodium falciparum parasitophorous vacuole antigens QF116 and exp-1/CRA are apparently identical. The epitope recognized by an inhibitory monoclonal antibody directed...

Kara, U.; Murray, B.; Pam, C.; Lahnstein, J.; Gould, H.; Kidson, C.; Saul, A., 1990: Chemical characterization of the parasitophorous vacuole membrane antigen QF 116 from Plasmodium falciparum. On the basis of amino acid sequencing and immunological cross-reactivity, the Plasmodium falciparum parasitophorous vacuole antigens QF116 and exp-1/CRA are apparently identical. The epitope recognized by an inhibitory monoclonal antibody directed...

Atkinson, C.T.; Hollingdale, M.R.; Aikawa, M., 1992: Localization of a 230-kD parasitophorous vacuole membrane antigen of Plasmodium berghei exoerythrocytic schizonts (LSA-2) by immunoelectron and confocal laser scanning microscopy. Using antiserum to a 230-kD parasitophorous vacuole membrane (PVM) antigen of Plasmodium berghei exoerythrocytic schizonts as a specific probe for the PVM, we studied the three-dimensional structure of this membrane within infected host cells by i...

Kara, U.; Pye, D.; Lord, R.; Pam, C.; Gould, H.; Geysen, M.; Jones, G.; Stenzel, D.; Kidson, C.; Saul, A., 1989: Immune response to a synthetic peptide corresponding to an epitope of a parasitophorous vacuole membrane antigen from Plasmodium falciparum. The parasitophorous vacuole membrane antigen QF 116 from Plasmodium falciparum contains a defined epitope, DNNLVSGP, proximal to the carboyxl-terminus which binds to the inhibitory monoclonal antibody 8E7/55. A synthetic peptide containing this ep...

Kara, U.A.; Stenzel, D.J.; Ingram, L.T.; Bushell, G.R.; Lopez, J.A.; Kidson, C., 1988: Inhibitory monoclonal antibody against a (myristylated) small-molecular-weight antigen from Plasmodium falciparum associated with the parasitophorous vacuole membrane. A small-molecular-weight antigen that occurs in asexual blood stages in synchronized cultures of Plasmodium falciparum was detected by a monoclonal antibody which inhibits parasite growth in vitro. This antigen, QF116, showed a molecular weight of...

Lanners, H.N.; Bafford, R.A.; Wiser, M.F., 1999: Characterization of the parasitophorous vacuole membrane from Plasmodium chabaudi and implications about its role in the export of parasite proteins. Little is known about how the malaria parasite transports and targets proteins into the host erythrocyte. Parasite proteins exported into the host cell not only have to cross the parasite plasma membrane but also must traverse the parasitophorous...

Lanners, H.N.; Bafford, R.A.; Wiser, M.F., 1999: Characterization of the parasitophorous vacuole membrane from Plasmodium chabaudi and implications about its role in the export of parasite proteins. Little is known about how the malaria parasite transports and targets proteins into the host erythrocyte. Parasite proteins exported into the host cell not only have to cross the parasite plasma membrane but also must traverse the parasitophorous...

Coppel, R.L.; Crewther, P.E.; Culvenor, J.G.; Perrin, L.H.; Brown, G.V.; Kemp, D.J.; Anders, R.F., 1988: Variation in p126, a parasitophorous vacuole antigen of Plasmodium falciparum. We describe a cDNA clone expressing part of p126, a parasitophorous vacuole antigen of Plasmodium falciparum that is processed to smaller fragments about the time of schizont rupture. The amino acid sequence includes determinants that are antigeni...