EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of a chloroplast coenzyme A-binding protein related to the peroxisomal thiolases


Plant Physiology 112(4): 1641-1647
Identification of a chloroplast coenzyme A-binding protein related to the peroxisomal thiolases
A 30-kD coenzyme A (CoA)-binding protein was isolated from spinach (Spinacea oleracea) chloroplast soluble extracts using affinity chromatography under conditions in which 95% of the total protein was excluded. The 30-kD protein contains an eight-amino-acid sequence, DVRLYYGA, that is identical to a region in a 36-kD protein of unknown function that is encoded by a kiwifruit (Actinidia deliciosa) cDNA. Southern blotting also detected a spinach gene that is related to the kiwifruit cDNA. The kiwifruit 36-kD protein that was synthesized in Escherichia coli was imported into chloroplasts and cleaved to a 30-kD form; it was processed to the same size in an organelle-free assay. Furthermore, the kiwifruit protein specifically bound to CoA. The kiwifruit protein contains a single cysteine within a domain that is related to the peroxisomal beta-ketoacyl-CoA thiolases, which catalyze the CoA-dependent degradative step of fatty acid beta-oxidation. Within 50 amino acids surrounding the cysteine, considered to be part of the thiolase active site, the kiwifruit protein shows approximately 26% sequence identity with the mango, cucumber, and rat peroxisomal thiolases. N-terminal alignment with these enzymes, relative to the cysteine, indicates that the 36-kD protein is cleaved after serine-58 during import, agreeing with the estimated size (approximately 6 kD) of a transit peptide. The 30-kD protein is also related to the E. coli and mitochondrial thiolases, as well as to the acetoacetyl-CoA thiolases of prokaryotes. Features distinguish it from members of the thiolase family, suggesting that it carries out a related but novel function. The protein is more distantly related to chloroplast beta-ketoacyl-acyl carrier protein synthase III, the initial condensing enzyme of fatty acid synthetase that utilizes acetyl-CoA.


Accession: 002863121

PMID: 8972603

DOI: 10.1104/pp.112.4.1641



Related references

Identification of the complementary dna clone which encodes the 58 kda protein containing the amino acid sequence of rat liver non specific lipid transfer protein sterol carrier protein 2 homology with rat peroxisomal and mitochondrial 3 oxoacyl coenzyme a thiolases. European Journal of Biochemistry 201(1): 233-240, 1991

Identification of the cDNA clone which encodes the 58-kDa protein containing the amino acid sequence of rat liver non-specific lipid-transfer protein (sterol-carrier protein 2). Homology with rat peroxisomal and mitochondrial 3-oxoacyl-CoA thiolases. European Journal of Biochemistry 201(1): 233-239, 1991

Peroxisomal multifunctional delta 3 delta 2 enoyl coenzyme a isomerase 2 enoyl coenzyme a hydratase and 3 hydroxyacyl coenzyme a dehydrogenase enzyme and peroxisomal bifunctional protein are the same protein in rat liver. Journal of Cell Biology 111(5 PART 2): 455A, 1990

An acyl-coenzyme A chain length dependent assay for 3-oxoacyl-coenzyme A thiolases employing acetyldithio-coenzyme A. Analytical Biochemistry 192(1): 49-54, 1991

The mechanism of inhibition of mitochondrial fatty acid oxidation by mercaptoacetate inhibition of acetoacetyl coenzyme a 2 methylacetoacetyl coenzyme a and 3 oxoacyl coenzyme a thiolases. Biochemical Society Transactions 14(4): 703-704, 1986

Recognition of a peroxisomal form of hydroxymethyl glutaryl coenzyme a reductase protein by an antibody that detects a peroxisomal targeting signal. Journal of Cell Biology 111(5 PART 2): 195A, 1990

Tissue specific induction of peroxisomal fatty acyl coenzyme a oxidase and peroxisomal bifunctional protein messenger rna levels by ciprofibrate. Journal Of Cell Biology Part 2: 66a, 1985

3-Oxoacyl-coenzyme A thiolases and L-3-hydroxyacyl-coenzyme A dehydrogenases. Biochemical Society Transactions 6(1): 80-83, 1978

Peroxisomal beta oxidation system of candida tropicalis purification of a multifunctional protein possessing enoyl coenzyme a hydratase ec 4.2.1.17 3 hydroxyacyl coenzyme a dehydrogenase ec 1.1.1.35 and 3 hydroxyacyl coenzyme a epimerase ec 5.1.2.3 activities. European Journal of Biochemistry 148(2): 285-292, 1985

The growing family of peroxisomal thiolases. Lipids 34 Suppl: S157-S157, 1999