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Identification of a major surface protein on Neospora caninum tachyzoites


, : Identification of a major surface protein on Neospora caninum tachyzoites. Parasitology Research. July; 826: 497-504

Neospora caninum is a recently identified coccidian parasite that is closely related to Toxoplasma gondii. Molecules associated with the surface of N. caninum tachyzoites are likely to be involved in the process of adhesion and invasion of host cells. They probably also participate in the interaction of the parasite with the immune system, and they could play an important role in the pathogenesis of the parasite. To identify such surface molecules, we performed subcellular fractionation studies of isolated N. caninum tachyzoites. Employing the nonionic detergent Triton-X-114, we prepared a membrane fraction. Immunoblot analysis of this fraction using polyclonal antisera directed against tachyzoites of N. caninum and T. gondii resulted in the identification of a protein of approximately 43 kDa (Nc-p43). This molecule was present in two isolates of Neospora (Nc-1 and Liverpool) but was absent in Toxoplasma (RH-strain) tachyzoites. Further immunofluorescence and immunogold transmission electron microscopy (TEM) studies using affinity-purified anti-Nc-p43 antibodies demonstrated the presence of this molecule on the surface of N. caninum tachyzoites.

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Accession: 002863168

PMID: 8832729

DOI: 10.1007/s004360050152

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Related references

Hemphill, A.; Gottstein, B., 1996: Identification of a major surface protein on Neospora caninum tachyzoites. Neospora caninum is a recently identified coccidian parasite that is closely related to Toxoplasma gondii. Molecules associated with the surface of N. caninum tachyzoites are likely to be involved in the process of adhesion and invasion of host ce...

Hemphill, A.; Gottstein, B., 1996: Identification of a major protein on Neospora caninum tachyzoites. Neospora caninum is a recently identified coccidian parasite that is closely related to Toxoplasma gondii. Molecules associated with the surface of N. caninum tachyzoites are likely to be involved in the process of adhesion and invasion of host ce...

Hemphill, A.F.chs, N.S.nda, S.G.ttstein, B.H.ntrich, B., 1997: Identification and partial charaterization of a 36 kDa surface protein on Neospora caninum tachyzoites. Parasitology 115(4): 371-380

Hemphill, A.; Fuchs, N.; Sonda, S.; Gottstein, B.; Hentrich, B., 1997: Identification and partial characterization of a 36 kDa surface protein on Neospora caninum tachyzoites. Neospora caninum, the causative agent of neosporosis, is a recently identified apicomplexan parasite which is structurally and biologically closely related to, but antigenically distinct from, Toxoplasma gondii. Molecules associated with the surfa...

Hemphill, A.G.jendran, N.S.nda, S.F.chs, N.G.ttstein, B.H.ntrich, B.J.nkins, M., 1998: Identification and characterization of a dense granule-associated protein in Neospora caninum tachyzoites. Neospora caninum is an apicomplexan parasite which is morphologically and ultrastructurally very similar to Toxoplasma gondii. In order to identify molecules involved in host cell entry and subsequent modification of the parasitophorous vacuole, a...

Hemphill, A.; Gajendran, N.; Sonda, S.; Fuchs, N.; Gottstein, B.; Hentrich, B.; Jenkins, M., 1998: Identification and characterisation of a dense granule-associated protein in Neospora caninum tachyzoites. Neospora caninum is an apicomplexan parasite which is morphologically and ultrastructurally very similar to Toxoplasma gondii. In order to identify molecules involved in host cell entry and subsequent modification of the parasitophorous vacuole, a...

Debache, K.; Guionaud, C.; Alaeddine, F.; Hemphill, A., 2010: Intraperitoneal and intra-nasal vaccination of mice with three distinct recombinant Neospora caninum antigens results in differential effects with regard to protection against experimental challenge with Neospora caninum tachyzoites. Recombinant NcPDI(recNcPDI), NcROP2(recNcROP2), and NcMAG1(recNcMAG1) were expressed in <i>Escherichia coli</i> and purified, and evaluated as potential vaccine candidates by employing the C57Bl/6 mouse cerebral infection model. Intrap...

Dijkstra, T.; Eysker, M.; Schares, G.; Conraths, F.J.; Wouda, W.; Barkema, H.W., 2001: Dogs shed Neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrum spiked with Neospora caninum tachyzoites. An experiment was carried out to determine whether bovine colostrum or placenta could be a source of infection of Neospora caninum for dogs. For this purpose, two dogs were fed bovine colostrum to which culture-derived N. caninum tachyzoites were...

Dijkstra, T.E.sker, M.S.hares, G.C.nraths, F.; Wouda, W.B.rkema, H., 2001: Dogs shed Neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrum spiked with Neospora caninum tachyzoites. An experiment was carried out to determine whether bovine colostrum or placenta could be a source of infection of Neospora caninum for dogs. For this purpose, two dogs were fed bovine colostrum to which culture-derived N. caninum tachyzoites were...

Naguleswaran, A.; Cannas, A.; Keller, N.; Vonlaufen, N.; Björkman, C.; Hemphill, A., 2002: Vero cell surface proteoglycan interaction with the microneme protein NcMIC(3) mediates adhesion of Neospora caninum tachyzoites to host cells unlike that in Toxoplasma gondii. Neospora caninum and Toxoplasma gondii are characterised by a very low host cell specificity, thus they are able to infect a wide range of different cells in vivo and in vitro. Infection of the host cell by tachyzoites is a process which is preced...